A New Platelet-Aggregation-Inhibiting Factor Isolated from Bothrops moojeni Snake Venom
Joint Authors
Barbosa de Sousa, Bruna
Mamede, Carla Cristine Neves
Matias, Mariana Santos
Pereira, Déborah Fernanda da Cunha
Dias, Edigar Henrique Vaz
Silva, Anielle Christine Almeida
Dantas, Noelio Oliveira
Costa, Júnia de Oliveira
Ribeiro de Queiroz, Mayara
de Oliveira, Fábio
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This work reports the purification and functional characterization of BmooPAi, a platelet-aggregation-inhibiting factor from Bothrops moojeni snake venom.
The toxin was purified by a combination of three chromatographic steps (ion-exchange on DEAE-Sephacel, molecular exclusion on Sephadex G-75, and affinity chromatography on HiTrap™ Heparin HP).
BmooPAi was found to be a single-chain protein with an apparent molecular mass of 32 kDa on 14% SDS-PAGE, under reducing conditions.
Sequencing of BmooPAi by Edman degradation revealed the amino acid sequence LGPDIVPPNELLEVM.
The toxin was devoid of proteolytic, haemorrhagic, defibrinating, or coagulant activities and induced no significant oedema or hyperalgesia.
BmooPAi showed a rather specific inhibitory effect on ristocetin-induced platelet aggregation in human platelet-rich plasma, whereas it had little or no effect on platelet aggregation induced by collagen and adenosine diphosphate.
The results presented in this work suggest that BmooPAi is a toxin comprised of disintegrin-like and cysteine-rich domains, originating from autolysis/proteolysis of PIII SVMPs from B.
moojeni snake venom.
This toxin may be of medical interest because it is a platelet aggregation inhibitor, which could potentially be developed as a novel therapeutic agent to prevent and/or treat patients with thrombotic disorders.
American Psychological Association (APA)
Barbosa de Sousa, Bruna& Mamede, Carla Cristine Neves& Matias, Mariana Santos& Pereira, Déborah Fernanda da Cunha& Ribeiro de Queiroz, Mayara& Dias, Edigar Henrique Vaz…[et al.]. 2017. A New Platelet-Aggregation-Inhibiting Factor Isolated from Bothrops moojeni Snake Venom. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136975
Modern Language Association (MLA)
Barbosa de Sousa, Bruna…[et al.]. A New Platelet-Aggregation-Inhibiting Factor Isolated from Bothrops moojeni Snake Venom. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1136975
American Medical Association (AMA)
Barbosa de Sousa, Bruna& Mamede, Carla Cristine Neves& Matias, Mariana Santos& Pereira, Déborah Fernanda da Cunha& Ribeiro de Queiroz, Mayara& Dias, Edigar Henrique Vaz…[et al.]. A New Platelet-Aggregation-Inhibiting Factor Isolated from Bothrops moojeni Snake Venom. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136975
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136975