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Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium
Joint Authors
Paul, Jonathan W.
Ilicic, Marina
Zakar, Tamas
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-02
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Background.
Regulation of myometrial progesterone receptor (PR) expression is an unresolved issue central to understanding the mechanism of functional progesterone withdrawal and initiation of labor in women.
Objectives.
To determine whether pregnant human myometrium undergoes culture-induced changes in PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture.
Methods.
Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF2α, NF-κB inhibitors, or TSA.
Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR.
PR-A/PR-B ratios were calculated.
Results.
PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture.
Steroids prevented the culture-induced increase in PR-T and PR-A expression.
Stretch blocked the effects of steroids on PR-T and PR-A expression.
PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio.
Conclusion.
Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype.
TSA maintained the nonlaboring PR isoform expression pattern.
This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.
American Psychological Association (APA)
Ilicic, Marina& Zakar, Tamas& Paul, Jonathan W.. 2017. Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium. BioMed Research International،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1137152
Modern Language Association (MLA)
Ilicic, Marina…[et al.]. Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium. BioMed Research International No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1137152
American Medical Association (AMA)
Ilicic, Marina& Zakar, Tamas& Paul, Jonathan W.. Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1137152
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137152