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Comparison between Peritoneal Macrophage Activation by Bougainvillea xbuttiana Extract and LPS andor Interleukins
Joint Authors
Abarca-Vargas, Rodolfo
Arteaga Figueroa, Lluvia
García Alanis, Claudia
Petricevich, Vera L.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Activation of macrophages may be one of the possible approaches in modulating inflammation.
We previously reported that Bougainvillea xbuttiana extract showed an immunomodulatory activity.
Here we compare the activation of macrophages exposed to B.
xbuttiana extract and compare it with the other treatments such as LPS, IL-4, and IL-10.
The cytotoxic effect of extract on peritoneal macrophages was determined by the technique of violet crystal staining.
To verify the activation of macrophages we used the tests of vacuolization, hydrogen peroxide production, and percentages of cellular expansion and phagocytosis.
The levels of interleukins secreted by macrophages treated with the extract, LPS, and cytokines were determined by the biological assay for the determination of TNF levels and by ELISA for all other interleukins.
NO levels were evaluated by colorimetric reactions using Griess reagent.
Our results showed that B.
xbuttiana extract induced (a) low cytotoxicity percentages, (b) increased vacuolization, hydrogen peroxide production and cell expansion and phagocytosis percentages, and (c) decreased production of TNF-α, IFN-γ, IL-1β, and IL-6 and potentiated production of IL-4, IL-10 and TGF-β.
These results suggest that B.
xbuttiana extract was able to activate the murine macrophages in a manner similar to those macrophages exposed to IL-4 and IL-10.
American Psychological Association (APA)
Arteaga Figueroa, Lluvia& Abarca-Vargas, Rodolfo& García Alanis, Claudia& Petricevich, Vera L.. 2017. Comparison between Peritoneal Macrophage Activation by Bougainvillea xbuttiana Extract and LPS andor Interleukins. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137170
Modern Language Association (MLA)
Arteaga Figueroa, Lluvia…[et al.]. Comparison between Peritoneal Macrophage Activation by Bougainvillea xbuttiana Extract and LPS andor Interleukins. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137170
American Medical Association (AMA)
Arteaga Figueroa, Lluvia& Abarca-Vargas, Rodolfo& García Alanis, Claudia& Petricevich, Vera L.. Comparison between Peritoneal Macrophage Activation by Bougainvillea xbuttiana Extract and LPS andor Interleukins. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137170
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137170