Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis
Joint Authors
Chandrasekharan, N. Vishvanath
Wickramasinghe, Gammadde Hewa Ishan Maduka
Rathnayake, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika
Weerasinghe, Mahindagoda Siril Samantha
Wijesundera, Ravindra Lakshman Chundananda
Wijesundera, Wijepurage Sandhya Sulochana
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
It is essential that major carbohydrate polymers in the lignocellulosic biomass are converted into fermentable sugars for the economical production of energy.
Xylan, the major component of hemicelluloses, is the second most naturally abundant carbohydrate polymer comprising 20–40% of the total biomass.
Endoxylanase (EXN) hydrolyzes xylan into mixtures of xylooligosaccharides.
The objective of this study was to genetically modify Pichia stipitis, a pentose sugar fermenting yeast species, to hydrolyze xylan into xylooligosaccharides via cloning and heterologous extracellular expression of EXNI gene from locally isolated Trichoderma virens species.
Pichia stipitis was engineered to carry the EXNI gene of T.
virens using pGAPZα expression vector.
The open reading frame encodes 191 amino acids and SDS-PAGE analysis revealed a 24 kDA recombinant protein.
The EXNI activity expressed by recombinant P.
stipitis clone under standard conditions using 1% beechwood xylan was 31.7 U/ml.
Molecular docking and molecular dynamics simulations were performed to investigate EXNI-xylan interactions.
Free EXNI and xylan bound EXNI exhibited similar stabilities and structural behavior in aqueous medium.
Furthermore, this in silico work opens avenues for the development of newer generation EXN proteins that can perform better and have enhanced catalytic activity.
American Psychological Association (APA)
Wickramasinghe, Gammadde Hewa Ishan Maduka& Rathnayake, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika& Chandrasekharan, N. Vishvanath& Weerasinghe, Mahindagoda Siril Samantha& Wijesundera, Ravindra Lakshman Chundananda& Wijesundera, Wijepurage Sandhya Sulochana. 2017. Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137281
Modern Language Association (MLA)
Wickramasinghe, Gammadde Hewa Ishan Maduka…[et al.]. Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137281
American Medical Association (AMA)
Wickramasinghe, Gammadde Hewa Ishan Maduka& Rathnayake, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika& Chandrasekharan, N. Vishvanath& Weerasinghe, Mahindagoda Siril Samantha& Wijesundera, Ravindra Lakshman Chundananda& Wijesundera, Wijepurage Sandhya Sulochana. Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137281
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137281