In Vitro Anticancer Potential of Berberis lycium Royle Extracts against Human Hepatocarcinoma (HepG2) Cells
Joint Authors
Song, Yuanda
Mustafa, Kiren
Mohamed, Hassan
Shah, Aabid Manzoor
Yu, Shaoxuan
Akhlaq, Muhammad
Xiao, Haifang
Li, Shaoqi
Naz, Tahira
Nosheen, Shaista
Bai, Xueyuan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Human liver cancer has emerged as a serious health concern in the world, associated with poorly available therapies.
The Berberis genus contains vital medicinal plants with miraculous healing properties and a wide range of bioactivities.
In this study, different crude extracts of B.
lycium Royle were prepared and screened against Human Hepatocarcinoma (HepG2) cell lines.
The water/ethanolic extract of B.
lycium Royle (BLE) exhibited significant antiproliferative activity against the HepG2 cancer cell line with an IC50 value of 47 μg/mL.
The extract decreased the clonogenic potential of HepG2 cells in a dose-dependent manner.
It induced apoptotic cell death in HepG2 cells that were confirmed by cytometric analysis and microscopic examination of cellular morphology through DAPI-stained cells.
Biochemical evidence of apoptosis came from elevating the intracellular ROS level that was accompanied by the loss of mitochondrial membrane potential.
The mechanism of apoptosis was further confirmed by gene expression analysis using RT-qPCR that revealed the decline in Bcl-2 independent of p53 mRNA and a rise in CDK1 while downregulating CDK5, CDK9, and CDK10 mRNA levels at 48 h of BLE treatment.
The most active fraction was subjected to HPLC which indicated the presence of berberine (48 μg/mL) and benzoic acid (15.8 μg/mL) as major compounds in BLE and a trace amount of luteolin, rutin, and gallic acid.
Our study highlighted the importance of the most active BLE extract as an excellent source of nutraceuticals against Human Hepatocarcinoma that can serve as an herbal natural cure against liver cancer.
American Psychological Association (APA)
Mustafa, Kiren& Mohamed, Hassan& Shah, Aabid Manzoor& Yu, Shaoxuan& Akhlaq, Muhammad& Xiao, Haifang…[et al.]. 2020. In Vitro Anticancer Potential of Berberis lycium Royle Extracts against Human Hepatocarcinoma (HepG2) Cells. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137376
Modern Language Association (MLA)
Mustafa, Kiren…[et al.]. In Vitro Anticancer Potential of Berberis lycium Royle Extracts against Human Hepatocarcinoma (HepG2) Cells. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1137376
American Medical Association (AMA)
Mustafa, Kiren& Mohamed, Hassan& Shah, Aabid Manzoor& Yu, Shaoxuan& Akhlaq, Muhammad& Xiao, Haifang…[et al.]. In Vitro Anticancer Potential of Berberis lycium Royle Extracts against Human Hepatocarcinoma (HepG2) Cells. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137376
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137376