A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples
Joint Authors
Patterson, Aileen P.
Gupta, Shivani
Taylor, Tracy
Liang, Binhua
Bullard, Jared
Sandstrom, Paul
Van Domselaar, Gary
Ji, Hezhao
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The prevalence of drug resistance (DR) mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV), supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research.
Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR) and reverse transcriptase (RT) regions of HIV-1 pol gene across many HIV-1 subtypes from LLV plasma samples.
HIV-spiked plasma samples from the External Quality Assurance Program Oversight Laboratory (EQAPOL), covering various HIV-1 subtypes, as well as clinical specimens were used to optimize and validate the protocol.
Our results demonstrate that this protocol has a broad HIV-1 subtype coverage and viral load span with high sensitivity and reproducibility.
Moreover, the protocol is robust even when plasma sample volumes are limited, the HIV viral load is unknown, and/or the HIV subtype is undetermined.
Thus, the protocol is applicable for the initial amplification of the HIV-1 PR and RT genes required for subsequent genotypic DR assays.
American Psychological Association (APA)
Gupta, Shivani& Taylor, Tracy& Patterson, Aileen P.& Liang, Binhua& Bullard, Jared& Sandstrom, Paul…[et al.]. 2017. A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples. BioMed Research International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1137382
Modern Language Association (MLA)
Gupta, Shivani…[et al.]. A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples. BioMed Research International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1137382
American Medical Association (AMA)
Gupta, Shivani& Taylor, Tracy& Patterson, Aileen P.& Liang, Binhua& Bullard, Jared& Sandstrom, Paul…[et al.]. A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1137382
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137382