Long Noncoding RNA NEAT1 Regulates TGF-β2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34aSnail1 and miR-204Zeb1 Pathways
Author
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-01
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
The aim of this study was to explore whether the long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1)/miR-34a/Snail1 and NEAT1/miR-204/Zeb1 pathways are involved in epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs).
Primary human LECs (HLECs) were separated and cultured.
Our results identified that TGF-β2 induces NEAT1 overexpression in a dose-dependent manner and a time-dependent manner.
Additionally, TGF-β2 induced downregulation of E-cadherin and upregulation of fibronectin in primary HLECs through a NEAT1-dependent mechanism.
Microarray analysis showed that NEAT1 overexpression inhibited the miR-34a and miR-204 levels in the LECs.
The expression of miR-34a and miR-204 was decreased, and the levels of Snail1 and Zeb1 were elevated in human posterior capsule opacification- (PCO-) attached LECs and the LECs obtained from anterior subcapsular cataract (ASC) by quantitative RT-PCR (qRT-PCR).
Mechanistic studies revealed that NEAT1 negatively regulates miR-34a or miR-204, and miR-34a or miR-204 directly targets Snail1 or Zeb1 by luciferase assay and RNA-binding protein immunoprecipitation assay, respectively.
Overall, the NEAT1/miR-34a/Snail1 and NEAT1/miR-204/Zeb1 pathways are involved in TGF-β2-induced EMT of HLECs.
In summary, TGF-β2 induces NEAT1 overexpression, which in turn suggests that NEAT1 acts as a ceRNA targeting Snail1 or Zeb1 by binding miR-34a or miR-204, and promotes the progression of EMT of LECs.
American Psychological Association (APA)
Dong, Ning. 2020. Long Noncoding RNA NEAT1 Regulates TGF-β2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34aSnail1 and miR-204Zeb1 Pathways. BioMed Research International،Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1137415
Modern Language Association (MLA)
Dong, Ning. Long Noncoding RNA NEAT1 Regulates TGF-β2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34aSnail1 and miR-204Zeb1 Pathways. BioMed Research International No. 2020 (2020), pp.1-19.
https://search.emarefa.net/detail/BIM-1137415
American Medical Association (AMA)
Dong, Ning. Long Noncoding RNA NEAT1 Regulates TGF-β2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34aSnail1 and miR-204Zeb1 Pathways. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1137415
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137415