PRDX2 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer In Vitro and In Vivo

Joint Authors

Chen, Yun
Yang, Sifu
Zhou, Hongying
Su, Dan

Source

BioMed Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-27

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Purpose.

Previous studies have reported that the levels of PRDX2 were correlated with tumorigenicity, recurrence, and prognosis of patients with different cancers.

We investigated the association between PRDX2 levels and the prognosis of lung cancer patients.

We also measured PRDX2 expression of non-small cell lung cancer (NSCLC) cells and examined its roles in the proliferation and migration in vitro and in vivo.

Methods.

We used the Kaplan–Meier plotter to analyze the survival of different levels of PRDX2 in lung cancer patients.

The expression of PRDX2 in normal bronchial epithelial cell line and NSCLC cell lines was measured by qRT-PCR and western blot assays.

Biological functions of NSCLC cells were detected by CCK8 and Transwell assays.

We constructed tumor growth model using subcutaneously injection of nude mice and metastasis model by tail vein injection in vivo.

The protein levels of proliferation related markers were measured by immunohistochemistry assay.

Immunofluorescence method was used to detected EMT-related proteins.

Results.

The high levels of PRDX2 were associated with bad prognosis in lung cancer patients, especially in patients with adenocarcinoma.

The expression of PRDX2 in NSCLC cell lines was higher than normal bronchial epithelial cells.

Knockdown of PRDX2 inhibited the proliferation, migration, and invasion in A549 cells, while overexpression of PRDX2 promoted the malignancy in NCI-H1299 cells in vitro.

Silencing PRDX2 restrained tumor growth and repressed lung metastasis by EMT in vivo.

Conclusion.

Our data indicates that PRDX2 functions as a protumor regulator and is involved in tumorigenesis and tumor progression of lung cancer.

American Psychological Association (APA)

Chen, Yun& Yang, Sifu& Zhou, Hongying& Su, Dan. 2020. PRDX2 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer In Vitro and In Vivo. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137417

Modern Language Association (MLA)

Chen, Yun…[et al.]. PRDX2 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer In Vitro and In Vivo. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1137417

American Medical Association (AMA)

Chen, Yun& Yang, Sifu& Zhou, Hongying& Su, Dan. PRDX2 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer In Vitro and In Vivo. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137417

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1137417