Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP
Joint Authors
Li, Hao
Yan, Lei
Su, Liang
Li, Rui
Bai, Jianrong
Sun, Feng-Jie
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Phosphate (Pi) deficiency in soil can have severe impacts on the growth, development, and production of maize worldwide.
In this study, a cDNA-sequence-related amplified polymorphism (cDNA-SRAP) transcript profiling technique was used to evaluate the gene expression in leaves and roots of maize under Pi stress for seven days.
A total of 2494 differentially expressed fragments (DEFs) were identified in response to Pi starvation with 1202 and 1292 DEFs in leaves and roots, respectively, using a total of 60 primer pairs in the cDNA-SRAP analysis.
These DEFs were categorized into 13 differential gene expression patterns.
Results of sequencing and functional analysis showed that 63 DEFs (33 in leaves and 30 in roots) were annotated to a total of 54 genes involved in diverse groups of biological pathways, including metabolism, photosynthesis, signal transduction, transcription, transport, cellular processes, genetic information, and organismal system.
This study demonstrated that (1) the cDNA-SRAP transcriptomic profiling technique is a powerful method to analyze differential gene expression in maize showing advantageous features among several transcriptomic methods; (2) maize undergoes a complex adaptive process in response to low Pi stress; and (3) a total of seven differentially expressed genes were identified in response to low Pi stress in leaves or roots of maize and could be used in the genetic modification of maize.
American Psychological Association (APA)
Yan, Lei& Su, Liang& Li, Rui& Li, Hao& Bai, Jianrong& Sun, Feng-Jie. 2020. Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1137450
Modern Language Association (MLA)
Yan, Lei…[et al.]. Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1137450
American Medical Association (AMA)
Yan, Lei& Su, Liang& Li, Rui& Li, Hao& Bai, Jianrong& Sun, Feng-Jie. Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1137450
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137450