Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells
Joint Authors
Zou, Hongjun
Song, Zhiwen
Liu, Jinbo
Tan, Chunye
Yu, Changlin
Xu, Xu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Yes-associated protein (YAP) is proved to increase miR-29a in the present study, but the relevant molecular mechanism is not clear.
Also, growing evidence indicates that the high-level miR-29a promotes the neurite outgrowth by decreasing PTEN (phosphatase and tensin homologue deleted on chromosome 10).
Results show that the expression of miR-29a increases but the PTEN decreases during transfecting the N2a cells with the YAP plasmid.
Meanwhile, the advancement of neurite outgrowth is presented via using multiple methods to detect the expression of GAP-43 and NF-200, which have a strong association with neurite outgrowth.
The expression of miR-29a, GAP-43, and NF-200 shows an opposite tendency compared to the PTEN when YAP is downregulated.
By treating N2a cells with miR-29a mimic and inhibitor, we also find the same conclusion.
For in silico analysis of miR-29a, its promoter may have a binding site for YAP.
Based on a luciferase reporter assay and a chromatin immunoprecipitation (ChIP) experiment, we demonstrate that YAP could increase the expression of miR-29a by targeting the promoter of miR-29a.
In conclusion, the results identify that YAP promotes the neurite outgrowth via targeting the promoter of miR-29a, and it may be an effective therapeutic medicine for the neural disease.
American Psychological Association (APA)
Tan, Chunye& Yu, Changlin& Song, Zhiwen& Zou, Hongjun& Xu, Xu& Liu, Jinbo. 2017. Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1137509
Modern Language Association (MLA)
Tan, Chunye…[et al.]. Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1137509
American Medical Association (AMA)
Tan, Chunye& Yu, Changlin& Song, Zhiwen& Zou, Hongjun& Xu, Xu& Liu, Jinbo. Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1137509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137509