Towards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes
Joint Authors
Menzel, Sarah
Finocchiaro, Nicole
Donay, Christine
Thiebes, Anja Lena
Hesselmann, Felix
Arens, Jutta
Djeljadini, Suzana
Wessling, Matthias
Schmitz-Rode, Thomas
Jockenhoevel, Stefan
Cornelissen, Christian Gabriel
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In patients with respiratory failure, extracorporeal lung support can ensure the vital gas exchange via gas permeable membranes but its application is restricted by limited long-term stability and hemocompatibility of the gas permeable membranes, which are in contact with the blood.
Endothelial cells lining these membranes promise physiological hemocompatibility and should enable prolonged application.
However, the endothelial cells increase the diffusion barrier of the blood-gas interface and thus affect gas transfer.
In this study, we evaluated how the endothelial cells affect the gas exchange to optimize performance while maintaining an integral cell layer.
Human umbilical vein endothelial cells were seeded on gas permeable cell culture membranes and cultivated in a custom-made bioreactor.
Oxygen transfer rates of blank and endothelialized membranes in endothelial culture medium were determined.
Cell morphology was assessed by microscopy and immunohistochemistry.
Both setups provided oxygenation of the test fluid featuring small standard deviations of the measurements.
Throughout the measuring range, the endothelial cells seem to promote gas transfer to a certain extent exceeding the blank membranes gas transfer performance by up to 120%.
Although the underlying principles hereof still need to be clarified, the results represent a significant step towards the development of a biohybrid lung.
American Psychological Association (APA)
Menzel, Sarah& Finocchiaro, Nicole& Donay, Christine& Thiebes, Anja Lena& Hesselmann, Felix& Arens, Jutta…[et al.]. 2017. Towards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137511
Modern Language Association (MLA)
Menzel, Sarah…[et al.]. Towards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137511
American Medical Association (AMA)
Menzel, Sarah& Finocchiaro, Nicole& Donay, Christine& Thiebes, Anja Lena& Hesselmann, Felix& Arens, Jutta…[et al.]. Towards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137511