Mechanism of Abnormal Chondrocyte Proliferation Induced by Piezo1-siRNA Exposed to Mechanical Stretch
Joint Authors
Sun, Yi
Leng, Ping
Li, Dawei
Gao, Huanshen
Li, Zhenghui
Li, Chenkai
Zhang, Haining
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To investigate the effect of small interfering RNA targeting mechanosensitive ion channel protein Piezo1 (Piezo1-siRNA) on abnormal chondrocyte proliferation exposed to mechanical stretch.
Methods.
Construct and screen effective Piezo1-siRNA sequences and explore an appropriate method to transfect lentiviral vector into chondrocytes exposed to mechanical stretch.
Western blot and RT-PCR were used to detect the mRNA and protein expression of Piezo1, Kif18A, and β-tubulin, respectively.
Flow cytometry was used to measure the changes in the chondrocyte cycle.
The proliferation of chondrocyte was evaluated by cell counting kit-8.
Results.
According to the mRNA and protein expression of Piezo1, the effective siRNA sequence was successfully screened.
Compared with the 0 h group, mechanical stretch upregulated the expression of Piezo1, Kif18A, and β-tubulin, resulting in chondrocyte cycle arrest and eventually inhibiting chondrocyte proliferation.
Moreover, Piezo1-siRNA transfection effectively blocks this process and promotes the proliferation of chondrocyte.
Conclusion.
Piezo1-siRNA can reduce the inhibition of chondrocyte proliferation induced by mechanical stretch via downregulating the expression of Kif18A and inhibiting the depolymerization of microtubules.
Piezo1-siRNA plays a protective role in chondrocytes, which provides a potential method for the treatment of OA under abnormal mechanical stimulation.
American Psychological Association (APA)
Sun, Yi& Leng, Ping& Li, Dawei& Gao, Huanshen& Li, Zhenghui& Li, Chenkai…[et al.]. 2020. Mechanism of Abnormal Chondrocyte Proliferation Induced by Piezo1-siRNA Exposed to Mechanical Stretch. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137513
Modern Language Association (MLA)
Sun, Yi…[et al.]. Mechanism of Abnormal Chondrocyte Proliferation Induced by Piezo1-siRNA Exposed to Mechanical Stretch. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1137513
American Medical Association (AMA)
Sun, Yi& Leng, Ping& Li, Dawei& Gao, Huanshen& Li, Zhenghui& Li, Chenkai…[et al.]. Mechanism of Abnormal Chondrocyte Proliferation Induced by Piezo1-siRNA Exposed to Mechanical Stretch. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137513
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137513