Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression
Joint Authors
Saadeldin, Islam M.
Kim, Geon A.
Jin, Jun-Xue
Lee, Sanghoon
Taweechaipaisankul, Anukul
Oh, Hyun Ju
Hwang, Joing-Ik
Ahn, Curie
Lee, Byeong Chun
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Soluble human tumor necrosis factor (shTNFRI-Fc) and human heme oxygenase 1 (hHO-1) are key regulators for protection against oxidative and inflammatory injury for xenotransplantation.
Somatic cells with more than 10 copy numbers of shTNFRI-Fc and hHO-1 were employed in somatic cell nuclear transfer to generate cloned pigs, thereby resulting in seven cloned piglets.
However, produced piglets were all dead within 24 hours after birth.
Obviously, postnatal death with liver apoptosis was reported in the higher copy number of shTNFRI-Fc and hHO-1 piglets.
In liver, the transcript levels of ferritin heavy chain, light chain, transferrin, and inducible nitric oxide synthase were significantly highly expressed compared to those of lower copy number of shTNFRI-Fc and hHO-1 piglets (P<0.05).
Also, H2O2 contents were increased, and superoxide dismutase was significantly lower in the higher copy number of shTNFRI-Fc and hHO-1 piglets (P<0.05).
These results indicate that TNFRI-Fc and hHO-1 overexpression may apparently induce free iron in the liver and exert oxidative stress by enhancing reactive oxygen species production and block normal postneonatal liver metabolism.
American Psychological Association (APA)
Kim, Geon A.& Jin, Jun-Xue& Lee, Sanghoon& Taweechaipaisankul, Anukul& Oh, Hyun Ju& Hwang, Joing-Ik…[et al.]. 2017. Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137525
Modern Language Association (MLA)
Kim, Geon A.…[et al.]. Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1137525
American Medical Association (AMA)
Kim, Geon A.& Jin, Jun-Xue& Lee, Sanghoon& Taweechaipaisankul, Anukul& Oh, Hyun Ju& Hwang, Joing-Ik…[et al.]. Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137525