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A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis
Joint Authors
Huang, He
Sun, Lei
He, Haiyang
Xia, Tianbao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In this report, we describe the development and testing of a new coated plate which improves the sensitivity and accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS).
The coated plate was covered with a thin layer of hydrophobic silicon dioxide, which enabled sample enrichment due to the water repellent nature of the silicon dioxide surface.
Sensitivity and required laser strengths were tested using peptide standards, with the results that these coated plates required lower laser power and showed increased sensitivity than that of common plates.
Accuracy was tested using bacteria, saliva, and serum samples.
The coated plates showed significantly increased degrees of accuracy through their capacity to reduce mass shift.
The importance and necessity of accuracy analysis in the assessment of new sample plates, which is rarely described in other papers, is also discussed.
American Psychological Association (APA)
Huang, He& Sun, Lei& He, Haiyang& Xia, Tianbao. 2020. A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis. BioMed Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1137546
Modern Language Association (MLA)
Huang, He…[et al.]. A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis. BioMed Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1137546
American Medical Association (AMA)
Huang, He& Sun, Lei& He, Haiyang& Xia, Tianbao. A New Silicon Dioxide-Coated MALDI-ToF Sample Plate for Peptide Analysis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1137546
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137546