Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering
Joint Authors
Shen, Yu
Bao, Xiaoming
Du, Binghai
Ding, Yanqin
Wang, Chengqiang
Zhao, Jianzhi
Qiu, Chenxi
Wang, Shihao
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Efficient and cost-effective fuel ethanol production from lignocellulosic materials requires simultaneous cofermentation of all hydrolyzed sugars, mainly including D-glucose, D-xylose, and L-arabinose.
Saccharomyces cerevisiae is a traditional D-glucose fermenting strain and could utilize D-xylose and L-arabinose after introducing the initial metabolic pathways.
The efficiency and simultaneous coutilization of the two pentoses and D-glucose for ethanol production in S.
cerevisiae still need to be optimized.
Previously, we constructed an L-arabinose-utilizing S.
cerevisiae BSW3AP.
In this study, we further introduced the XI and XR-XDH metabolic pathways of D-xylose into BSW3AP to obtain D-glucose, D-xylose, and L-arabinose cofermenting strain.
Benefits of evolutionary engineering: the resulting strain BSW4XA3 displayed a simultaneous coutilization of D-xylose and L-arabinose with similar consumption rates, and the D-glucose metabolic capacity was not decreased.
After 120 h of fermentation on mixed D-glucose, D-xylose, and L-arabinose, BSW4XA3 consumed 24% more amounts of pentoses and the ethanol yield of mixed sugars was increased by 30% than that of BSW3AP.
The resulting strain BSW4XA3 was a useful chassis for further enhancing the coutilization efficiency of mixed sugars for bioethanol production.
American Psychological Association (APA)
Wang, Chengqiang& Zhao, Jianzhi& Qiu, Chenxi& Wang, Shihao& Shen, Yu& Du, Binghai…[et al.]. 2017. Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137557
Modern Language Association (MLA)
Wang, Chengqiang…[et al.]. Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137557
American Medical Association (AMA)
Wang, Chengqiang& Zhao, Jianzhi& Qiu, Chenxi& Wang, Shihao& Shen, Yu& Du, Binghai…[et al.]. Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137557
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137557