BASP1 Suppresses Cell Growth and Metastasis through Inhibiting Wntβ-Catenin Pathway in Gastric Cancer
Joint Authors
Li, Li
Meng, Qinghua
Li, Guoying
Zhao, Limei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
Our research is designed to explore the function of brain acid soluble protein 1 (BASP1) in the progression of gastric cancer (GC) and its underlying molecular mechanisms.
Methods.
In this study, the expression of BASP1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in both GC tissue and GC cells.
The cell cloning, proliferation, apoptosis, migration, and invasion potential of AGS and HGC-27 cells were, respectively, determined using colony formation assay, 5-ethynyl-20-deoxyuridine (EDU) assay, flow cytometry, and Transwell assay.
The protein expressions of Bax, caspase-3, Bcl-2, matrix metalloproteinases 2 (MMP-2), MMP-9, Wilms tumor 1 (WT1), Wnt, and β-catenin in AGS and HGC-27 cells were measured by western blot.
In addition, the mRNA expressions of WT1, Wnt, and β-catenin in AGS and HGC-27 cells were detected by qRT-PCR.
Results.
BASP1 expression was significantly downregulated in both GC tissue and GC cells.
BASP1 overexpression markedly repressed proliferation, migration, and invasion and facilitated apoptosis in AGS and HGC-27 cells.
In addition, BASP1 overexpression notably promoted the protein expression of Bax and caspase-3 in AGS and HGC-27 cells and inhibited the expression of Bcl-2, MMP-2, and MMP-9.
Moreover, BASP1 overexpression significantly inhibited the mRNA and protein expression of WT1, Wnt, and β-catenin in AGS and HGC-27 cells.
Conclusion.
BASP1 could significantly suppress cell proliferation, migration, and invasion and promote apoptosis through inhibiting the activation of the Wnt/β-catenin pathway in GC.
American Psychological Association (APA)
Li, Li& Meng, Qinghua& Li, Guoying& Zhao, Limei. 2020. BASP1 Suppresses Cell Growth and Metastasis through Inhibiting Wntβ-Catenin Pathway in Gastric Cancer. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137566
Modern Language Association (MLA)
Li, Li…[et al.]. BASP1 Suppresses Cell Growth and Metastasis through Inhibiting Wntβ-Catenin Pathway in Gastric Cancer. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1137566
American Medical Association (AMA)
Li, Li& Meng, Qinghua& Li, Guoying& Zhao, Limei. BASP1 Suppresses Cell Growth and Metastasis through Inhibiting Wntβ-Catenin Pathway in Gastric Cancer. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137566
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137566