Inflammatory Factors Induce Thrombosis through the miR-146b-3pp38MAPKCOX-2 Pathway
Joint Authors
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
Inflammatory responses play important roles in the pathogenesis of atherosclerosis.
The purpose of this study was to investigate the relationship between microRNA-146b-3p (miR-146b-3p) and inflammatory factors in thrombosis.
Method.
THP-1 cells were cultured in vitro, Western blot was used to determine the protein levels of COX-2 and p38MAPK in the cells, and real-time PCR was used to detect the mRNA expression of miRNA-146b-3p and COX-2.
A lentiviral expression vector of miRNA-146b-3p and its inhibitor were constructed to transfect THP-1 cells.
COX-2 and p38MAPK expression in transfected cells was detected by Western blot and real-time PCR, respectively.
Results.
Ang II and TNF-α could elevate the expression of COX-2 in monocytes.
The expression of COX-2 was upregulated by p38MAPK, which could be phosphorylated by Ang II, while there was an increasing tendency of p38MAPK phosphorylation after TNF-α stimulation.
In addition, COX-2 expression and P38MAPK phosphorylation could be downregulated by miRNA-146b-3p and upregulated by the miRNA-146b-3p inhibitor.
Ang II could increase miR-146b-3p expression, although there was no significant difference; however, the expression of miR-146b-3p was enhanced significantly by TNF-α.
Conclusion.
Our data implied that altered expression of miR-146b-3p was closely related to the progression of inflammation mediating the P38MAPK/COX-2 pathway.
We suggest that the miR-146b-3p/p38MAPK/COX-2 pathway plays a key role in inflammation and arterial thrombosis.
American Psychological Association (APA)
Su, Zhengjia& Wu, Fang. 2020. Inflammatory Factors Induce Thrombosis through the miR-146b-3pp38MAPKCOX-2 Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1137614
Modern Language Association (MLA)
Su, Zhengjia& Wu, Fang. Inflammatory Factors Induce Thrombosis through the miR-146b-3pp38MAPKCOX-2 Pathway. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1137614
American Medical Association (AMA)
Su, Zhengjia& Wu, Fang. Inflammatory Factors Induce Thrombosis through the miR-146b-3pp38MAPKCOX-2 Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1137614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137614