Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance
Joint Authors
Wang, Yanli
Diao, Sijun
Hu, Maoqing
Zhang, Lin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The Tsc1-mTOR signaling pathway is often related to obesity, and epigenetic modification may lead to expression changes of obesity-related gene.
Therefore, we aim to investigate the methylation of the Tsc1-mTOR signaling pathway in regulation of obesity susceptibility.
Wistar rats were fed a normal diet or a high-fat diet to develop animal models.
Protein and mRNA expression levels of Tsc1-mTOR signaling in the hypothalamus were determined by Western blot and quantitative real-time PCR.
Methylation of Tsc1 gene promoter was detected by bisulfite genomic sequence.
Both mRNA and protein expression levels of Tsc1 in DIO group hypothalamus were lower; mTOR and its downstream targets S6K1, 4EBP1, and S6 protein expression levels were higher than those of the DIO-R group and the chow group.
The Tsc1 gene promoter methylation rate in the hypothalamus was 92.05±3.07% in the DIO group, 87.27±1.91% in the DIO-R group, and 88.18%±3.20% in the chow group, respectively, with significantly higher levels in the DIO group.
Both the expression levels of Tsc1 gene promoter methylation and Tsc1-mTOR signaling pathway in the hypothalamus of DIO rats and DIO-R rats are different.
These findings may shed light on the potential mechanism for the differentiation of obesity susceptibility.
American Psychological Association (APA)
Wang, Yanli& Diao, Sijun& Hu, Maoqing& Zhang, Lin. 2020. Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance. BioMed Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1137615
Modern Language Association (MLA)
Wang, Yanli…[et al.]. Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance. BioMed Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1137615
American Medical Association (AMA)
Wang, Yanli& Diao, Sijun& Hu, Maoqing& Zhang, Lin. Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1137615
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137615