Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance

Joint Authors

Wang, Yanli
Diao, Sijun
Hu, Maoqing
Zhang, Lin

Source

BioMed Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-30

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Medicine

Abstract EN

The Tsc1-mTOR signaling pathway is often related to obesity, and epigenetic modification may lead to expression changes of obesity-related gene.

Therefore, we aim to investigate the methylation of the Tsc1-mTOR signaling pathway in regulation of obesity susceptibility.

Wistar rats were fed a normal diet or a high-fat diet to develop animal models.

Protein and mRNA expression levels of Tsc1-mTOR signaling in the hypothalamus were determined by Western blot and quantitative real-time PCR.

Methylation of Tsc1 gene promoter was detected by bisulfite genomic sequence.

Both mRNA and protein expression levels of Tsc1 in DIO group hypothalamus were lower; mTOR and its downstream targets S6K1, 4EBP1, and S6 protein expression levels were higher than those of the DIO-R group and the chow group.

The Tsc1 gene promoter methylation rate in the hypothalamus was 92.05±3.07% in the DIO group, 87.27±1.91% in the DIO-R group, and 88.18%±3.20% in the chow group, respectively, with significantly higher levels in the DIO group.

Both the expression levels of Tsc1 gene promoter methylation and Tsc1-mTOR signaling pathway in the hypothalamus of DIO rats and DIO-R rats are different.

These findings may shed light on the potential mechanism for the differentiation of obesity susceptibility.

American Psychological Association (APA)

Wang, Yanli& Diao, Sijun& Hu, Maoqing& Zhang, Lin. 2020. Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance. BioMed Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1137615

Modern Language Association (MLA)

Wang, Yanli…[et al.]. Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance. BioMed Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1137615

American Medical Association (AMA)

Wang, Yanli& Diao, Sijun& Hu, Maoqing& Zhang, Lin. Methylation of Hypothalamic Tsc1-mTOR Signaling in Regulation of Obesity and Obesity Resistance. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1137615

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1137615