Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
Joint Authors
Caselli, C.
Parodi, Oberdan
Rocchiccioli, Silvia
Pelosi, Gualtiero
Bastiani, Luca
Sbrana, Silverio
Clemente, Alberto
Cecchettini, Antonella
Ceccherini, Elisa
Neglia, Danilo
Chiappino, Dante
Smit, Jeff M.
Scholte, Arthur J.
Campolo, Jonica
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background and Aims.
Atherosclerosis is an inflammatory disease with long-lasting activation of innate immunity and monocytes are the main blood cellular effectors.
We aimed to investigate monocyte phenotype (subset fraction and marker expression) at different stages of coronary atherosclerosis in stable coronary artery disease (CAD) patients.
Methods.
73 patients with chronic coronary syndrome were evaluated by CT coronary angiography (CTCA) and classified by maximal diameter stenosis of major vessels into three groups of CAD severity: CAD1 (no CAD/minimal CAD, n°=30), CAD2 (non-obstructive CAD, n°=21), and CAD3 (obstructive CAD, n°=22).
Flow cytometry for CD14, CD16, and CCR2 was used to quantify Mon1, Mon2, and Mon3 subsets.
Expression of CD14, CD16, CD18, CD11b, HLA-DR, CD163, CCR2, CCR5, CX3CR1, and CXCR4 was also measured.
Adhesion molecules and cytokines were quantified by ELISA.
Results.
Total cell count and fraction of Mon2 were higher in CAD2 and CAD3 compared to CAD1.
By multivariate regression analysis, Mon2 cell fraction and Mon2 expression of CX3CR1, CD18, and CD16 showed a statistically significant and independent increase, parallel to stenosis severity, from CAD1 to CAD2 and CAD3 groups.
A similar trend was also present for CX3CR1 and HLA-DR expressions on total monocyte population.
A less calcified plaque composition was associated to a higher Mon2 expression of CD16 and higher TNF-α levels.
IL-10 levels were lower at greater stenosis severity, while the IFN-γ/IL-10 ratio, a marker of a systemic pro-inflammatory imbalance, was directly correlated to stenosis degree and number of noncalcified plaques.
Conclusions.
The results of this study suggest that a specific pattern of inflammation-correlated monocyte marker expression is associated to higher stenosis severity and less calcified lesions in stable CAD.
The clinical trial Identifier is NCT04448691.
American Psychological Association (APA)
Sbrana, Silverio& Campolo, Jonica& Clemente, Alberto& Bastiani, Luca& Cecchettini, Antonella& Ceccherini, Elisa…[et al.]. 2020. Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1137631
Modern Language Association (MLA)
Sbrana, Silverio…[et al.]. Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1137631
American Medical Association (AMA)
Sbrana, Silverio& Campolo, Jonica& Clemente, Alberto& Bastiani, Luca& Cecchettini, Antonella& Ceccherini, Elisa…[et al.]. Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1137631
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137631