Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen
Joint Authors
Li, Guiyu
Peng, Yue
Zhao, Tiejian
Lin, Jiyong
Duan, Xuelin
Wei, Yanfei
Ma, Jing
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Critical roles for liver sinusoidal endothelial cells (LSECs) in liver fibrosis have been demonstrated, while little is known regarding the underlying molecular mechanisms of drugs delivered to the LSECs.
Our previous study revealed that plumbagin plays an antifibrotic role in liver fibrosis.
In this study, we investigated whether plumbagin alleviates capillarization of hepatic sinusoids by downregulating endothelin-1 (ET-1), vascular endothelial growth factor (VEGF), laminin (LN), and type IV collagen on leptin-stimulated LSECs.
We found that normal LSECs had mostly open fenestrae and no organized basement membrane.
Leptin-stimulated LSECs showed the formation of a continuous basement membrane with few open fenestrae, which were the features of capillarization.
Expression of ET-1, VEGF, LN, and type IV collagen was enhanced in leptin-stimulated LSECs.
Plumbagin was used to treat leptin-stimulated LSECs.
The sizes and numbers of open fenestrae were markedly decreased, and no basement membrane production was found after plumbagin administration.
Plumbagin decreased the levels of ET-1, VEGF, LN, and type IV collagen in leptin-stimulated LSECs.
Plumbagin promoted downregulation of ET-1, VEGF, LN, and type IV collagen mRNA.
Altogether, our data reveal that plumbagin reverses capillarization of hepatic sinusoids by downregulation of ET-1, VEGF, LN, and type IV collagen.
American Psychological Association (APA)
Li, Guiyu& Peng, Yue& Zhao, Tiejian& Lin, Jiyong& Duan, Xuelin& Wei, Yanfei…[et al.]. 2017. Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1137669
Modern Language Association (MLA)
Li, Guiyu…[et al.]. Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1137669
American Medical Association (AMA)
Li, Guiyu& Peng, Yue& Zhao, Tiejian& Lin, Jiyong& Duan, Xuelin& Wei, Yanfei…[et al.]. Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1137669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137669