The Effect of Lecithins Coupled Decorin Nanoliposomes on Treatment of Carbon Tetrachloride-Induced Liver Fibrosis
Joint Authors
Zhu, Yiping
Chen, Guojun
Guo, Junping
Liang, Xiao
Wang, Xianfa
Yu, Weihua
Zhu, Linghua
Ma, Rui
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study aimed to investigate the effect of bile duct-targeting lecithins- (PC-) coupled decorin (DCN) (PC-DCN) nanoliposomes against liver fibrosis in vitro and in vivo.
We prepared PC-DCN nanoliposomes by using rat astrocytes, HSC-T6, to verify the antifibrosis effect of PC-DCN in vitro.
First, we established a rat model of carbon tetrachloride-induced fibrosis.
PC-DCN nanoliposomes were then injected into fibrotic rats via the portal vein or bile duct.
The EdU assay was performed to analyze cell proliferation.
Immunofluorescence staining was used to detect α-smooth muscle actin (α-SMA) expression.
Western blot was performed to examine the expression of α-SMA, collagen type I alpha 1 (COL1A1), and transforming growth factor-β (TGF-β) protein.
The levels of aspartate transaminase (AST), alanine transaminase (ALT), and total bilirubin (TBIL) were examined by enzyme-linked immunosorbent assay (ELISA) analysis.
Hematoxylin and eosin (H&E) staining and Masson trichrome staining were used to determine liver tissue lesions and liver fibrosis.
Compared with TGF-β group, PC-DCN treatment could significantly reduce cell proliferation.
Western blot analysis indicated that the expression of α-SMA, COL1A1, and TGF-β was downregulated after treatment with PC-DCN in vitro and in vivo.
Immunofluorescence staining confirmed that α-SMA expression was reduced by PC-DCN.
Furthermore, H&E staining and Masson trichrome staining showed that the administration of PC-DCN nanoliposomes via the bile duct could reduce the extent of liver fibrosis.
PCR analysis showed that PC-DCN administration could reduce proinflammatory cytokines IL-6, TNF-α, and IL-1β expression via the bile duct.
The administration of PC-DCN nanoliposomes also significantly downregulated liver function indicators ALT, AST, and TBIL.
The results of our study indicated that PC-DCN could effectively reduce the extent of liver fibrosis.
American Psychological Association (APA)
Chen, Guojun& Zhu, Yiping& Liang, Xiao& Wang, Xianfa& Yu, Weihua& Guo, Junping…[et al.]. 2020. The Effect of Lecithins Coupled Decorin Nanoliposomes on Treatment of Carbon Tetrachloride-Induced Liver Fibrosis. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137682
Modern Language Association (MLA)
Chen, Guojun…[et al.]. The Effect of Lecithins Coupled Decorin Nanoliposomes on Treatment of Carbon Tetrachloride-Induced Liver Fibrosis. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1137682
American Medical Association (AMA)
Chen, Guojun& Zhu, Yiping& Liang, Xiao& Wang, Xianfa& Yu, Weihua& Guo, Junping…[et al.]. The Effect of Lecithins Coupled Decorin Nanoliposomes on Treatment of Carbon Tetrachloride-Induced Liver Fibrosis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137682
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137682