Bioavailability and Pharmacokinetics of Anisatin in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
Joint Authors
Ma, Jianshe
Bao, Xi
Jiang, Xiajuan
Wang, Xianqin
Zhou, Quan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Anisatin is a neurotoxic sesquiterpene dilactone wildly found in plants of the family Illiciaceae.
Due to morphological similarities among Illiciaceae fruits, fatal poisonings are frequent.
Objective.
This study is aimed at developing a rapid, simple ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine anisatin’s bioavailability in mouse blood and the method’s application to pharmacokinetics.
Methods.
Blood samples were preprocessed by protein precipitation using acetonitrile.
Salicin (internal standard, IS) and anisatin were gradient-eluted by a mobile phase of methanol and water (0.1% formic acid) in a UPLC BEH C18 column.
This step involved using an electrospray ionization source of anisatin at a mass-to-charge ratio (m/z) of 327.1→127.0 and IS at m/z 285.1→122.9 in the negative ion mode with multiple reaction monitoring.
Results.
The calibration curve ranged from 1 to 2000 ng/ml (r>0.995), with the method’s accuracy ranging from 86.3% to 106.9%.
Intraday and interday precision were lower than 14%, and the matrix effect was between 93.9% and 103.3%.
The recovery rate was higher than 67.2%.
Conclusions.
The developed UPLC-MS/MS method was successfully used for a pharmacokinetic study of oral (1 mg/kg) and intravenous (0.5 mg/kg) administration of anisatin to mice—the absolute bioavailability of anisatin in the mouse blood was 22.6%.
American Psychological Association (APA)
Bao, Xi& Jiang, Xiajuan& Ma, Jianshe& Wang, Xianqin& Zhou, Quan. 2020. Bioavailability and Pharmacokinetics of Anisatin in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1137724
Modern Language Association (MLA)
Bao, Xi…[et al.]. Bioavailability and Pharmacokinetics of Anisatin in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1137724
American Medical Association (AMA)
Bao, Xi& Jiang, Xiajuan& Ma, Jianshe& Wang, Xianqin& Zhou, Quan. Bioavailability and Pharmacokinetics of Anisatin in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1137724
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137724