Ketamine and Propofol Protect Neuron Cells from Oxygen-Glucose Deprivation-Induced Injury through SAPKJNK Signalling Pathway
Joint Authors
Qi, Aihua
Cao, Yiyun
Wang, Aizhong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Ketamine and propofol are commonly used anaesthetic reagents.
Recent research revealed that ketamine and propofol have an important role in cell survival.
However, it remains unknown whether they affect the outcome of hypoxic-ischemic brain injury.
To address this issue, we in this study investigated the effects of ketamine and propofol on the survival and proliferation of neuronal PC12 cells after exposure to oxygen-glucose deprivation- (OGD-) induced injury.
PC12 cells were maintained under a 3-dimensional (3D) culture system to mimic a real physiological microenvironment.
The cell injury was induced by 5% CO2 and 95% N2 for a different time point.
MTT assay was used for the cell proliferation assay.
The cell apoptosis was evaluated by annexin V and propidium iodide (PI) labeling, immunofluorescence staining, transmission electron microscopy (TEM), flow cytometry, and Western blot, respectively.
Our results showed that PC12 cell apoptosis was significantly increased for up to 70% after the cells were treated with OGD for 24 hours and reduced to baseline at the 72-hour time point.
However, pretreatment with ketamine and propofol significantly protected the cells from OGD-induced cell apoptosis, as evidenced by more expression of antiapoptotic Bcl-2 and lower expression of proapoptotic cleaved caspase-3, phosphor-SAPK/JNK, and phosphor-c-Jun than those of untreated control cells.
Thus, we conclude that ketamine and propofol protected PC12 cells from OGD-induced cell apoptosis, at least partially through the SAPK/JNK signalling pathway.
American Psychological Association (APA)
Qi, Aihua& Cao, Yiyun& Wang, Aizhong. 2020. Ketamine and Propofol Protect Neuron Cells from Oxygen-Glucose Deprivation-Induced Injury through SAPKJNK Signalling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1137797
Modern Language Association (MLA)
Qi, Aihua…[et al.]. Ketamine and Propofol Protect Neuron Cells from Oxygen-Glucose Deprivation-Induced Injury through SAPKJNK Signalling Pathway. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1137797
American Medical Association (AMA)
Qi, Aihua& Cao, Yiyun& Wang, Aizhong. Ketamine and Propofol Protect Neuron Cells from Oxygen-Glucose Deprivation-Induced Injury through SAPKJNK Signalling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1137797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137797