Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study
Joint Authors
Sinauridze, Elena I.
Ataullakhanov, Fazoil I.
Alexandrovich, Yuliya G.
Kosenko, Elena A.
Obydennyi, Sergey I.
Kaminsky, Yuriy G.
Kireev, Igor I.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Erythrocytes (RBCs) loaded with alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALD) can metabolize plasma ethanol and acetaldehyde but with low efficiency.
We investigated the rate-limiting factors in ethanol oxidation by these enzymes loaded into RBCs.
Mathematical modeling and in vitro experiments on human RBCs loaded simultaneously with ADH and ALD (by hypoosmotic dialysis) were performed.
The simulation showed that the rate of nicotinamide-adenine dinucleotide (NAD+) generation in RBC glycolysis, but not the activities of the loaded enzymes, is the rate-limiting step in external ethanol oxidation.
The rate of oxidation could be increased if RBCs are supplemented by NAD+ and pyruvate.
Our experimental data verified this theoretical conclusion.
RBCs loaded with the complete system of ADH, ALD, NAD+, and pyruvate metabolized ethanol 20–40 times faster than reported in previous studies.
The one-step procedure of hypoosmotic dialysis is the optimal method to encapsulate ADH and ALD in RBCs after cell recovery, encapsulation yield, osmotic resistance, and RBC-indexes.
Consequently, transfusion of the RBCs loaded with the complete metabolic system, including ADH, ALD, pyruvate, and NAD+ in the patients with alcohol intoxication, may be a promising method for rapid detoxification of blood alcohol based on metabolism.
American Psychological Association (APA)
Alexandrovich, Yuliya G.& Kosenko, Elena A.& Sinauridze, Elena I.& Obydennyi, Sergey I.& Kireev, Igor I.& Ataullakhanov, Fazoil I.…[et al.]. 2017. Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study. BioMed Research International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1137813
Modern Language Association (MLA)
Alexandrovich, Yuliya G.…[et al.]. Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study. BioMed Research International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1137813
American Medical Association (AMA)
Alexandrovich, Yuliya G.& Kosenko, Elena A.& Sinauridze, Elena I.& Obydennyi, Sergey I.& Kireev, Igor I.& Ataullakhanov, Fazoil I.…[et al.]. Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1137813
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137813