Magnetic Separation Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Various Types of Matrices: A Review
Joint Authors
Slana, Iva
Husakova, Marketa
Dziedzinska, Radka
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-31
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The main reasons to improve the detection of Mycobacterium avium subsp.
paratuberculosis (MAP) are animal health and monitoring of MAP entering the food chain via meat, milk, and/or dairy products.
Different approaches can be used for the detection of MAP, but the use of magnetic separation especially in conjunction with PCR as an end-point detection method has risen in past years.
However, the extraction of DNA which is a crucial step prior to PCR detection can be complicated due to the presence of inhibitory substances.
Magnetic separation methods involving either antibodies or peptides represent a powerful tool for selective separation of target bacteria from other nontarget microorganisms and inhibitory sample components.
These methods enable the concentration of pathogens present in the initial matrix into smaller volume and facilitate the isolation of sufficient quantities of pure DNA.
The purpose of this review was to summarize the methods based on the magnetic separation approach that are currently available for the detection of MAP in a broad range of matrices.
American Psychological Association (APA)
Husakova, Marketa& Dziedzinska, Radka& Slana, Iva. 2017. Magnetic Separation Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Various Types of Matrices: A Review. BioMed Research International،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1137824
Modern Language Association (MLA)
Husakova, Marketa…[et al.]. Magnetic Separation Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Various Types of Matrices: A Review. BioMed Research International No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1137824
American Medical Association (AMA)
Husakova, Marketa& Dziedzinska, Radka& Slana, Iva. Magnetic Separation Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Various Types of Matrices: A Review. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1137824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137824