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Renoprotective Effects of Aldose Reductase Inhibitor Epalrestat against High Glucose-Induced Cellular Injury
Joint Authors
El Gamal, Heba
Eid, Ali Hussein
Munusamy, Shankar
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Diabetic nephropathy (DN) is the leading cause of end stage renal disease worldwide.
Increased glucose flux into the aldose reductase (AR) pathway during diabetes was reported to exert deleterious effects on the kidney.
The objective of this study was to investigate the renoprotective effects of AR inhibition in high glucose milieu in vitro.
Rat renal tubular (NRK-52E) cells were exposed to high glucose (30 mM) or normal glucose (5 mM) media for 24 to 48 hours with or without the AR inhibitor epalrestat (1 μM) and assessed for changes in Akt and ERK1/2 signaling, AR expression (using western blotting), and alterations in mitochondrial membrane potential (using JC-1 staining), cell viability (using MTT assay), and cell cycle.
Exposure of NRK-52E cells to high glucose media caused acute activation of Akt and ERK pathways and depolarization of mitochondrial membrane at 24 hours.
Prolonged high glucose exposure (for 48 hours) induced AR expression and G1 cell cycle arrest and decreased cell viability (84% compared to control) in NRK-52E cells.
Coincubation of cells with epalrestat prevented the signaling changes and renal cell injury induced by high glucose.
Thus, AR inhibition represents a potential therapeutic strategy to prevent DN.
American Psychological Association (APA)
El Gamal, Heba& Eid, Ali Hussein& Munusamy, Shankar. 2017. Renoprotective Effects of Aldose Reductase Inhibitor Epalrestat against High Glucose-Induced Cellular Injury. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137833
Modern Language Association (MLA)
El Gamal, Heba…[et al.]. Renoprotective Effects of Aldose Reductase Inhibitor Epalrestat against High Glucose-Induced Cellular Injury. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137833
American Medical Association (AMA)
El Gamal, Heba& Eid, Ali Hussein& Munusamy, Shankar. Renoprotective Effects of Aldose Reductase Inhibitor Epalrestat against High Glucose-Induced Cellular Injury. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137833
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137833