![](/images/graphics-bg.png)
Integrated Analysis of Hub Genes and miRNAs in Dilated Cardiomyopathy
Joint Authors
Huang, Kai
Wen, Shuyan
Huang, Jiechun
Wang, Fangrui
Pang, Liewen
Wang, Yiqing
Sun, Xiaotian
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-15
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Purpose.
The aim of this study is to identify hub genes and miRNAs by the miRNA-mRNA interaction network in dilated cardiomyopathy (DCM) disease.
Methods.
The differentially expressed miRNAs (DEMis) and mRNAs (DEMs) were selected using data of DCM patients downloaded from the GEO database (GSE112556 and GSE3585).
Gene Ontology (GO) pathway analysis and transcription factor enrichment analysis were used for selecting DEMis, and the target mRNAs of DEMis were filtered by using miRDB, miRTarBase, and TargetScan.
Cytoscape software was used to visualize the network between miRNAs and mRNAs and calculate the hub genes.
GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the mRNAs in the regulatory network.
Results.
A total of 9 DEMis and 281 DEMs were selected, from which we reconstructed the miRNA-mRNA network consisting of 7 miRNAs and 51 mRNAs.
The top 10 nodes, miR-144-3p, miR-363-3p, miR-9-3p, miR-21-3p, miR-144-5p, miR-338-3p, ID4 (inhibitor of DNA binding/differentiation 4), miR-770-5p, PIK3R1 (p85α regulatory subunit of phosphoinositide 3-kinase (PI3K)), and FN1 (fibronectin 1), were identified as important regulators.
Conclusions.
The study uncovered several important hub genes and miRNAs involved in the pathogenesis of DCM, among which, the miR-144-3p/FN1 and miR-9-3p/FN1 pathways may play an important role in myocardial fibrosis, which can help identify the etiology of DCM, and provide potential therapeutic targets.
American Psychological Association (APA)
Huang, Kai& Wen, Shuyan& Huang, Jiechun& Wang, Fangrui& Pang, Liewen& Wang, Yiqing…[et al.]. 2020. Integrated Analysis of Hub Genes and miRNAs in Dilated Cardiomyopathy. BioMed Research International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1137862
Modern Language Association (MLA)
Huang, Kai…[et al.]. Integrated Analysis of Hub Genes and miRNAs in Dilated Cardiomyopathy. BioMed Research International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1137862
American Medical Association (AMA)
Huang, Kai& Wen, Shuyan& Huang, Jiechun& Wang, Fangrui& Pang, Liewen& Wang, Yiqing…[et al.]. Integrated Analysis of Hub Genes and miRNAs in Dilated Cardiomyopathy. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1137862
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137862