In Vivo Suppression of Autophagy via Lentiviral shRNA Targeting Atg5 Improves Lupus-Like Syndrome
Joint Authors
Sun, Kuang-Hui
Liu, Chi-Jui
Chou, Chun-Che
Sun, Guang-Huan
Tang, Shye-Jye
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In both mouse models and clinical patients with lupus, autophagy levels were significantly elevated and correlated with disease activity.
Furthermore, autophagy can promote the survival of B and T cells, plasma cell differentiation, and antibody production.
These results suggest that autophagy may promote the progression of lupus by regulating the survival of autoreactive immune cells.
Therefore, we aimed at studying whether suppressing autophagy can modulate lupus progression in vivo.
First, we found that the autophagy levels in splenocytes and lymphocytes of peripheral blood (PB) were elevated and positively correlated with disease severity in lupus-prone mice.
The shAtg5-lentivirus, which effectively inhibits autophagy in vitro, was then injected into the lupus-prone mice.
Autophagy levels in lymph node cells and PB lymphocytes were reduced following Atg5 suppression.
We also found that lymphadenopathy and the numbers of plasma cells, CD4-CD8-, and CD4+ T cells decreased in mice treated with the shAtg5-lentivirus.
The mice treated with shAtg5-lentivirus exhibited lower levels of proteinuria, serum anti-dsDNA antibody, B-cell activating factor (BAFF), and glomerular immune complex deposition.
Therefore, targeting autophagy to moderate overactivated autophagy in immune cells seems to be a novel strategy for combination therapy of lupus.
American Psychological Association (APA)
Liu, Chi-Jui& Tang, Shye-Jye& Chou, Chun-Che& Sun, Guang-Huan& Sun, Kuang-Hui. 2020. In Vivo Suppression of Autophagy via Lentiviral shRNA Targeting Atg5 Improves Lupus-Like Syndrome. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137884
Modern Language Association (MLA)
Liu, Chi-Jui…[et al.]. In Vivo Suppression of Autophagy via Lentiviral shRNA Targeting Atg5 Improves Lupus-Like Syndrome. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1137884
American Medical Association (AMA)
Liu, Chi-Jui& Tang, Shye-Jye& Chou, Chun-Che& Sun, Guang-Huan& Sun, Kuang-Hui. In Vivo Suppression of Autophagy via Lentiviral shRNA Targeting Atg5 Improves Lupus-Like Syndrome. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137884
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137884