The Potential Regulatory Roles of lncRNAs in DNA Damage Response in Human Lymphocytes Exposed to UVC Irradiation
Joint Authors
Sun, Yeqing
Xu, Dan
Wang, Yue
Wang, Jia
Qi, Fei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Long noncoding RNAs (lncRNAs) are a class of noncoding RNAs that modulate gene expression, thereby participating in the regulation of various cellular processes.
However, it is not clear about the expression and underlying mechanism of lncRNAs in irradiation-induced DNA damage response.
In the present study, we performed integrative analysis of lncRNA-mRNA expression profile in human lymphocytes irradiated with ultraviolet-C (UVC).
The results showed that exposure to UVC irradiation dose-dependently increased the fluorescence intensity of γ-H2AX and induced cell death.
Microarray analysis revealed that up-regulated lncRNAs were more common than down-regulated lncRNAs with the increase of radiation dose in UVC-radiated cells.
Stem analysis demonstrated the relationship between lncRNA expression level and radiation dose.
qPCR results confirmed that LOC338799 and its coexpressed genes such as LCE1F and ISCU showed the increase in expression levels with the increase of UVC radiation dose.
We utilized Cytoscape to screen out 5 lncRNAs and 13 coexpressed genes linking to p53, which might participate in the regulation of DNA damage, cell cycle arrest, apoptosis, and cell death.
These findings suggest that lncRNAs might play a role in UVC-induced DNA damage response through regulating expression of genes in p53 signaling pathway.
American Psychological Association (APA)
Xu, Dan& Wang, Yue& Wang, Jia& Qi, Fei& Sun, Yeqing. 2020. The Potential Regulatory Roles of lncRNAs in DNA Damage Response in Human Lymphocytes Exposed to UVC Irradiation. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137888
Modern Language Association (MLA)
Xu, Dan…[et al.]. The Potential Regulatory Roles of lncRNAs in DNA Damage Response in Human Lymphocytes Exposed to UVC Irradiation. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1137888
American Medical Association (AMA)
Xu, Dan& Wang, Yue& Wang, Jia& Qi, Fei& Sun, Yeqing. The Potential Regulatory Roles of lncRNAs in DNA Damage Response in Human Lymphocytes Exposed to UVC Irradiation. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137888