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Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3KAktGSK-3ββ-Catenin Signaling Pathway
Joint Authors
Song, Fanglong
Jiang, Dawei
Wang, Tianchen
Wang, Yi
Lou, Yi
Zhang, Yinquan
Ma, Hui
Kang, Yifan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Osteogenesis and adipogenesis of bone marrow mesenchymal stem cells (BMSCs) are regarded as being of great importance in the regulation of bone remodeling.
In this study, rat BMSCs were exposed to different levels of cyclic mechanical stress generated by liquid drops and cultured in general medium or adipogenic medium.
Markers of osteogenic (Runx2 and Collagen I) and adipogenic (C/EBPα, PPARγ, and lipid droplets) differentiation were detected using Western blot and histological staining.
The protein levels of members of the phosphatidylinositol 3-kinase (PI3K)/Akt/glycogen synthase kinase 3β (GSK-3β)/β-catenin signaling pathway were also examined.
Results showed that small-magnitude stress significantly upregulated Runx2 and Collagen I and downregulated PPARγ and C/EBPα expression in BMSCs cultured in adipogenic medium, while large-magnitude stress reversed the effect when compared with unloading groups.
The PI3K/Akt signaling pathway could be strongly activated by mechanical stimulation; however, large-magnitude stress led to decreased activation of the signaling pathway when compared with small-magnitude stress.
Activation of β-catenin with LiCl led to increased expression of Runx2 and Collagen I and reduction of C/EBPα and PPARγ expression in BMSCs.
Inhibition of PI3K/Akt signaling partially blocked the expression of β-catenin.
Taken together, our results indicate that mechanical stress-regulated osteogenesis and adipogenesis of rat BMSCs are mediated, at least in part, by the PI3K/Akt/GSK-3β/β-catenin signaling pathway.
American Psychological Association (APA)
Song, Fanglong& Jiang, Dawei& Wang, Tianchen& Wang, Yi& Lou, Yi& Zhang, Yinquan…[et al.]. 2017. Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3KAktGSK-3ββ-Catenin Signaling Pathway. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137904
Modern Language Association (MLA)
Song, Fanglong…[et al.]. Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3KAktGSK-3ββ-Catenin Signaling Pathway. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1137904
American Medical Association (AMA)
Song, Fanglong& Jiang, Dawei& Wang, Tianchen& Wang, Yi& Lou, Yi& Zhang, Yinquan…[et al.]. Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3KAktGSK-3ββ-Catenin Signaling Pathway. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137904
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137904