Challenges in Cancer Biomarker Discovery Exemplified by the Identification of Diagnostic MicroRNAs in Prostate Tissues
Joint Authors
Antoniewicz, Artur A.
Michal, Mikula
Jerzy, Ostrowski
Karczmarski, Jakub
Paziewska, Agnieszka
Ambrozkiewicz, Filip
Kulecka, Maria
Cybulska, Magdalena
Szymanski, Michal
Dobruch, Jakub
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-06
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Identification and clinical translation of routinely tested biomarkers require a complex and multistep workflow.
Here, we described a confirmatory process estimating the utility of previously identified candidate tissue miRNAs for diagnosis of prostate cancer (PCa).
RNA was isolated from formalin-fixed paraffin-embedded (FFPE) prostate tissue surgically resected from 44 patients with PCa and 24 patients with benign prostate hyperplasia (BPH).
Of the 92 RNA samples obtained, 68 represented 42 malignant (PCa) areas and 26 represented nonmalignant (PCa 0%) areas of the prostate tissue sections.
The levels of miR-32-5p, miR-183-5p, miR-141-5p, miR-187-3p, miR-375, miR-663b, miR-615-3p, miR-205-5p, miR-221-3p, and miR-222-3p were evaluated using Exiqon chemistry.
Five (miR-32-5p, miR-141-5p, miR-187-3p, miR-375, and miR-615-3p), one (miR-32-5p), and two (miR-32-5p and miR-141-5p) miRNAs discriminated between BPH and areas of cancer-bearing prostate tissue harboring different numbers of cancer cells (PCa 15–70%, PCa 2–10%, and PCA 0%, respectively), with an area under the receiver operating characteristics curve (AUC-ROC) > 0.9.
Only miRNA 32-5p discriminated BPH specimens from sections of cancer-bearing prostate tissue with a low percentage, a high percentage, or no dysplastic cells.
miR-32-5p could be considered as potential diagnostic biomarker discriminating BPH from noncancerous areas within cancer-bearing prostate tissue.
However, further clinical studies are warranted to confirm its diagnostic utility.
American Psychological Association (APA)
Ambrozkiewicz, Filip& Karczmarski, Jakub& Kulecka, Maria& Paziewska, Agnieszka& Cybulska, Magdalena& Szymanski, Michal…[et al.]. 2020. Challenges in Cancer Biomarker Discovery Exemplified by the Identification of Diagnostic MicroRNAs in Prostate Tissues. BioMed Research International،Vol. 2020, no. 2020, pp.1-4.
https://search.emarefa.net/detail/BIM-1137957
Modern Language Association (MLA)
Ambrozkiewicz, Filip…[et al.]. Challenges in Cancer Biomarker Discovery Exemplified by the Identification of Diagnostic MicroRNAs in Prostate Tissues. BioMed Research International No. 2020 (2020), pp.1-4.
https://search.emarefa.net/detail/BIM-1137957
American Medical Association (AMA)
Ambrozkiewicz, Filip& Karczmarski, Jakub& Kulecka, Maria& Paziewska, Agnieszka& Cybulska, Magdalena& Szymanski, Michal…[et al.]. Challenges in Cancer Biomarker Discovery Exemplified by the Identification of Diagnostic MicroRNAs in Prostate Tissues. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-4.
https://search.emarefa.net/detail/BIM-1137957
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137957