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Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTORTFEB Signaling Pathway
Joint Authors
Fu, Zhiling
Zhang, Ze
Wu, Xiuying
Zhang, Jin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Hydrogen-rich saline (HRS) has strong anti-inflammatory, antioxidative stress, and antiapoptotic properties.
The study focused on the protection of HRS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rat models and the relationship with autophagic regulation and mTOR/TFEB signaling pathway.
Material and Methods.
The LPS-induced ALI rats’ model was established.
Pathohistological change in lung tissue was detected by hematoxylin-eosin staining.
The inflammatory cytokines were examined by enzyme-linked immunosorbent assay (ELISA).
The key apoptosis proteins and autophagy-relevant proteins were analyzed by western blotting.
In vitro, HPMEC models of ALI were treated with LPS.
The inflammatory cytokines were detected.
Apoptosis rate was determined by flow cytometry.
The autophagy and mTOR/TFEB signaling pathway-related proteins were detected by western blot and immunohistochemical staining.
Results.
HRS attenuated LPS-induced ALI and apoptosis both in vivo and in vitro.
HRS attenuated inflammatory response, inhibited apoptosis, induced and activated autophagy in LPS-induced ALI model, and downregulated mTOR/TFEB signaling pathway.
The protection of HRS can be blocked by autophagy inhibitor.
Moreover, mTOR activator reversed HRS protection and mTOR inhibitor enhanced HRS protection in LPS-induced model and HRS activated autophagy via mTOR/TFEB signaling pathway.
Conclusion.
The results confirmed the protection of HRS in LPS-induced ALI by regulating apoptosis through inhibiting the mTOR/TFEB signaling pathway.
American Psychological Association (APA)
Fu, Zhiling& Zhang, Ze& Wu, Xiuying& Zhang, Jin. 2020. Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTORTFEB Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1137978
Modern Language Association (MLA)
Fu, Zhiling…[et al.]. Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTORTFEB Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1137978
American Medical Association (AMA)
Fu, Zhiling& Zhang, Ze& Wu, Xiuying& Zhang, Jin. Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTORTFEB Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1137978
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137978