Lutein plus Water Chestnut (Trapa bispinosa Roxb.) Extract Inhibits the Development of Cataracts and Induces Antioxidant Gene Expression in Lens Epithelial Cells
Joint Authors
Shibata, Teppei
Shibata, Shinsuke
Sasaki, Hiroshi
Kubo, Eri
Ishida, Hidetoshi
Tanaka, Yuki
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Age-related cataract (ARC) is the major cause of blindness worldwide.
The most significant factors are the maximal exposure of the eye lens to environmental stressors, including oxidative and glycative load.
The administration of antioxidant and antiglycative supplements may reduce the risk of cataract progression.
In this study, the effects of lutein (LU) and water chestnut (Trapa bispinosa Roxb.) extract (TBE) on cataracts and the expression of antioxidant-related genes were assessed in Shumiya cataract rats (SCRs).
LU+TBE or castor oil (COil) as a control was administered to 6- or 9-week-old cataractous SCRs and noncataractous SCRs via a feeding needle for 3 or 4 weeks.
Five-week-old SCRs were provided ad libitum access to solid regular chow containing LU, TBE, LU+TBE, or the same chow without LU and/or TBE for 3 weeks.
Lenses from all rats were then extracted and photographed.
The right eyes of the rats were processed for histological observation, and the left eyes were used for total RNA extraction from lens epithelial cells (LEC).
The mRNA levels of antioxidant proteins, peroxiredoxin 6, and catalase were examined using real-time quantitative polymerase chain reaction.
Lens opacity appeared in all cataractous SCRs that began receiving LU+TBE at 9 weeks of age.
However, compared to the COil group, lens opacity was decreased in the cataractous LU+TBE SCRs in all experiments.
The mRNA expression levels of peroxiredoxin 6 and catalase in LECs of cataractous SCRs and cultured human LECs increased after the administration of LU+TBE.
Collectively, our results highlight the anticataract and antioxidative effects of LT+TBE in SCRs.
LT+TBE supplementation may, thus, be useful in delaying cataract progression.
American Psychological Association (APA)
Ishida, Hidetoshi& Shibata, Teppei& Shibata, Shinsuke& Tanaka, Yuki& Sasaki, Hiroshi& Kubo, Eri. 2020. Lutein plus Water Chestnut (Trapa bispinosa Roxb.) Extract Inhibits the Development of Cataracts and Induces Antioxidant Gene Expression in Lens Epithelial Cells. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1138014
Modern Language Association (MLA)
Ishida, Hidetoshi…[et al.]. Lutein plus Water Chestnut (Trapa bispinosa Roxb.) Extract Inhibits the Development of Cataracts and Induces Antioxidant Gene Expression in Lens Epithelial Cells. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1138014
American Medical Association (AMA)
Ishida, Hidetoshi& Shibata, Teppei& Shibata, Shinsuke& Tanaka, Yuki& Sasaki, Hiroshi& Kubo, Eri. Lutein plus Water Chestnut (Trapa bispinosa Roxb.) Extract Inhibits the Development of Cataracts and Induces Antioxidant Gene Expression in Lens Epithelial Cells. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1138014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138014