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Txndc9 Is Required for Meiotic Maturation of Mouse Oocytes
Joint Authors
Yang, Li Guo
Ma, Fanhua
Hou, Liming
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Txndc9 (thioredoxin domain containing protein 9) has been shown to be involved in mammalian mitosis; however, its function in mammalian oocyte meiosis remains unclear.
In this study, we initially found that Txndc9 is expressed during meiotic maturation of mouse oocytes and higher expression of Txndc9 mRNA and protein occurred in germinal vesicle (GV) stage.
By using confocal scanning, we observed that Txndc9 localized at both nucleus and cytoplasm, especially at spindle microtubules.
Specific depletion of Txndc9 by siRNA in mouse oocyte resulted in decreasing the rate of first polar body extrusion and increasing abnormal spindle assemble.
Moreover, knockdown of Txndc9 in germinal vesicle (GV) stage oocytes led to higher level of reactive oxygen species (ROS) and lower level of antioxidant glutathione (GSH) as compared with control oocytes, which indicated that Txndc9 may be involved in mediating the redox balance.
In summary, our results demonstrated that Txndc9 is crucial for mouse oocyte maturation by regulating spindle assembly, polar body extrusion, and redox status.
American Psychological Association (APA)
Ma, Fanhua& Hou, Liming& Yang, Li Guo. 2017. Txndc9 Is Required for Meiotic Maturation of Mouse Oocytes. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138015
Modern Language Association (MLA)
Ma, Fanhua…[et al.]. Txndc9 Is Required for Meiotic Maturation of Mouse Oocytes. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1138015
American Medical Association (AMA)
Ma, Fanhua& Hou, Liming& Yang, Li Guo. Txndc9 Is Required for Meiotic Maturation of Mouse Oocytes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138015
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138015