![](/images/graphics-bg.png)
Self-Conjugation of the Enteropathogenic Escherichia coli Adherence Factor Plasmid of Four Typical EPEC Isolates
Joint Authors
Silva, Claudia
Zavala-Alvarado, Crispín
Puente, José L.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The enteropathogenic Escherichia coli (EPEC) adherence factor plasmid (pEAF) encodes the proteins involved in the biogenesis of the bundle-forming pilus (BFP), a key virulence factor that mediates microcolony formation and the localized adherence phenotype on the surface of the host enterocytes.
The presence or absence of this plasmid defines typical EPEC (tEPEC) and atypical EPEC (aEPEC), respectively.
Although lateral transfer of pEAF has been evidenced by phylogenetic studies, conjugal transfer ability has been experimentally established only for two pEAF plasmids from strains isolated in the late 60s.
In the present work, we tested the self-conjugation ability of four pEAF plasmids from tEPEC strains isolated between 2007 and 2008 from children in Peru and the potential of aEPEC to receive them.
A kanamycin resistance cassette was inserted into donor pEAF plasmids in order to provide a selectable marker in the conjugation experiments.
Two aEPEC isolated from the same geographic region were used as recipient strains along with the laboratory E.
coli DH5α strain.
Here we show that the four pEAF plasmids tested are self-conjugative, with transfer frequencies in the range of 10−6 to 10−9.
Moreover, the generation of aEPEC strains harboring pEAF plasmids provides valuable specimens to further perform functional studies.
American Psychological Association (APA)
Silva, Claudia& Zavala-Alvarado, Crispín& Puente, José L.. 2017. Self-Conjugation of the Enteropathogenic Escherichia coli Adherence Factor Plasmid of Four Typical EPEC Isolates. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1138047
Modern Language Association (MLA)
Silva, Claudia…[et al.]. Self-Conjugation of the Enteropathogenic Escherichia coli Adherence Factor Plasmid of Four Typical EPEC Isolates. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1138047
American Medical Association (AMA)
Silva, Claudia& Zavala-Alvarado, Crispín& Puente, José L.. Self-Conjugation of the Enteropathogenic Escherichia coli Adherence Factor Plasmid of Four Typical EPEC Isolates. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1138047
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138047