Dexmedetomidine Attenuates Lipopolysaccharide Induced MCP-1 Expression in Primary Astrocyte
Joint Authors
Liu, Huan
Davis, Jacques R. J.
Wu, Zhi-Lin
Faez Abdelgawad, Amro
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Neuroinflammation which presents as a possible mechanism of delirium is associated with MCP-1, an important proinflammatory factor which is expressed on astrocytes.
It is known that dexmedetomidine (DEX) possesses potent anti-inflammatory properties.
This study aimed to investigate the potential effects of DEX on the production of MCP-1 in lipopolysaccharide-stimulated astrocytes.
Materials and Methods.
Astrocytes were treated with LPS (10 ng/ml, 50 ng/ml, 100 ng/ml, and 1000 ng/ml), DEX (500 ng/mL), LPS (100 ng/ml), and DEX (10, 100, and 500 ng/mL) for a duration of three hours; expression levels of MCP-1 were measured by real-time PCR.
The double immunofluorescence staining protocol was utilized to determine the expression of α2-adrenoceptors (α2AR) and glial fibrillary acidic protein (GFAP) on astrocytes.
Results.
Expressions of MCP-1 mRNA in astrocytes were induced dose-dependently by LPS.
Administration of DEX significantly inhibited the expression of MCP-1 mRNA (P<0.001).
Double immunofluorescence assay showed that α2AR colocalize with GFAP, which indicates the expression of α2-adrenoceptors in astrocytes.
Conclusions.
DEX is a potent suppressor of MCP-1 in astrocytes induced with lipopolysaccharide through α2A-adrenergic receptors, which potentially explains its beneficial effects in the treatment of delirium by attenuating neuroinflammation.
American Psychological Association (APA)
Liu, Huan& Davis, Jacques R. J.& Wu, Zhi-Lin& Faez Abdelgawad, Amro. 2017. Dexmedetomidine Attenuates Lipopolysaccharide Induced MCP-1 Expression in Primary Astrocyte. BioMed Research International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1138062
Modern Language Association (MLA)
Liu, Huan…[et al.]. Dexmedetomidine Attenuates Lipopolysaccharide Induced MCP-1 Expression in Primary Astrocyte. BioMed Research International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1138062
American Medical Association (AMA)
Liu, Huan& Davis, Jacques R. J.& Wu, Zhi-Lin& Faez Abdelgawad, Amro. Dexmedetomidine Attenuates Lipopolysaccharide Induced MCP-1 Expression in Primary Astrocyte. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1138062
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138062