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Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure
Joint Authors
Mahara, Atsushi
Kakudo, Natsuko
Kusumoto, Kenji
Yamaoka, Tetsuji
Morimoto, Naoki
Notodihardjo, Sharon Claudia
Mitsui, Toshihito
Le, Tien Minh
Munisso, Maria Chiara
Moriyama, Mariko
Moriyama, Hiroyuki
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-31
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
High hydrostatic pressure (HHP) is a physical method for inactivating cells or tissues without using chemicals such as detergents.
We previously reported that HHP at 200 MPa for 10 min was able to inactivate all cells in skin and giant congenital melanocytic nevus (GCMN) without damaging the extracellular matrix.
We also reported that HHP at 150 MPa for 10 min was not sufficient to inactivate them completely, while HHP at 200 MPa for 10 min was able to inactivate them completely.
We intend to apply HHP to treat malignant skin tumor as the next step; however, the conditions necessary to kill each kind of cell have not been explored.
In this work, we have performed a detailed experimental study on the critical pressure and pressurization time using five kinds of human skin cells and skin tumor cells, including keratinocytes (HEKas), dermal fibroblasts (HDFas), adipose tissue-derived stem cells (ASCs), epidermal melanocytes (HEMa-LPs), and malignant melanoma cells (MMs), using pressures between 150 and 200 MPa.
We pressurized cells at 150, 160, 170, 180, or 190 MPa for 1 s, 2 min, and 10 min and evaluated the cellular activity using live/dead staining and proliferation assays.
The proliferation assay revealed that HEKas were inactivated at a pressure higher than 150 MPa and a time period longer than 2 min, HDFas and MMs were inactivated at a pressure higher than 160 MPa and for 10 min, and ASCs and HEMa-LPs were inactivated at a pressure higher than 150 MPa and for 10 min.
However, some HEMa-LPs were observed alive after HHP at 170 MPa for 10 min, so we concluded that HHP at a pressure higher than 180 MPa for 10 min was able to inactivate five kinds of cells completely.
American Psychological Association (APA)
Mitsui, Toshihito& Morimoto, Naoki& Mahara, Atsushi& Notodihardjo, Sharon Claudia& Le, Tien Minh& Munisso, Maria Chiara…[et al.]. 2020. Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure. BioMed Research International،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1138128
Modern Language Association (MLA)
Mitsui, Toshihito…[et al.]. Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure. BioMed Research International No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1138128
American Medical Association (AMA)
Mitsui, Toshihito& Morimoto, Naoki& Mahara, Atsushi& Notodihardjo, Sharon Claudia& Le, Tien Minh& Munisso, Maria Chiara…[et al.]. Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1138128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138128