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Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique
Joint Authors
He, Ke
Zhao, Ayong
Wang, Han
Shen, Zhonghao
Zhou, Xiaolong
Yang, Songbai
Yan, Feifei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The difference in muscle fiber types is very important to the muscle development and meat quality of broilers.
At present, the molecular regulation mechanisms of skeletal muscle fiber-type transformation in broilers are still unclear.
In this study, differentially expressed genes between breast and leg muscles in broilers were analyzed using RNA-seq.
A total of 767 DEGs were identified.
Compared with leg muscle, there were 429 upregulated genes and 338 downregulated genes in breast muscle.
Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in cellular processes, single organism processes, cells, and cellular components, as well as binding and catalytic activity.
KEGG analysis shows that a total of 230 DEGs were mapped to 126 KEGG pathways and significantly enriched in the four pathways of glycolysis/gluconeogenesis, starch and sucrose metabolism, insulin signalling pathways, and the biosynthesis of amino acids.
Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the differential expression of 7 selected DEGs, and the results were consistent with RNA-seq data.
In addition, the expression profile of MyHC isoforms in chicken skeletal muscle cells showed that with the extension of differentiation time, the expression of fast fiber subunits (types IIA and IIB) gradually increased, while slow muscle fiber subunits (type I) showed a downward trend after 4 days of differentiation.
The differential genes screened in this study will provide some new ideas for further understanding the molecular mechanism of skeletal muscle fiber transformation in broilers.
American Psychological Association (APA)
Wang, Han& Shen, Zhonghao& Zhou, Xiaolong& Yang, Songbai& Yan, Feifei& He, Ke…[et al.]. 2020. Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1138130
Modern Language Association (MLA)
Wang, Han…[et al.]. Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1138130
American Medical Association (AMA)
Wang, Han& Shen, Zhonghao& Zhou, Xiaolong& Yang, Songbai& Yan, Feifei& He, Ke…[et al.]. Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1138130
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138130