Multivalent Fusion DNA Vaccine against Brucella abortus
Joint Authors
Gómez, Leonardo
Llanos, Javiera
Escalona, Emilia
Sáez, Darwin
Álvarez, Francisco
Molina, Raúl
Flores, Manuel
Oñate, Angel
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
As an alternative brucellosis prevention method, we evaluated the immunogenicity induced by new multivalent DNA vaccines in BALB/c mice.
We constructed the vaccines by fusion of BAB1_0273 and/or BAB1_0278 open reading frames (ORFs) from genomic island 3 (GI-3) and the Brucella abortus 2308 sodC gene with a link based on prolines and alanines (pV273-sod, pV278-sod, and pV273-278-sod, resp.).
Results show that immunization with all tested multivalent DNA vaccines induced a specific humoral and cellular immune response.
These novel multivalent vaccines significantly increased the production of IgM, IgG, and IgG2a antibodies as well as IFN-γ levels and the lymphoproliferative response of splenocytes.
Although immunization with these multivalent vaccines induced a typical T-helper 1- (Th1-) dominated immune response, such immunogenicity conferred low protection levels in mice challenged with the B.
abortus 2308 pathogenic strain.
Our results demonstrated that the expression of BAB1_0273 and/or BABl_0278 antigens conjugated to SOD protein can polarize mice immunity to a Th1-type phenotype, conferring low levels of protection.
American Psychological Association (APA)
Gómez, Leonardo& Llanos, Javiera& Escalona, Emilia& Sáez, Darwin& Álvarez, Francisco& Molina, Raúl…[et al.]. 2017. Multivalent Fusion DNA Vaccine against Brucella abortus. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138142
Modern Language Association (MLA)
Gómez, Leonardo…[et al.]. Multivalent Fusion DNA Vaccine against Brucella abortus. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1138142
American Medical Association (AMA)
Gómez, Leonardo& Llanos, Javiera& Escalona, Emilia& Sáez, Darwin& Álvarez, Francisco& Molina, Raúl…[et al.]. Multivalent Fusion DNA Vaccine against Brucella abortus. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138142