LncRNA HOTAIR Contributes to Sorafenib Resistance through Suppressing miR-217 in Hepatic Carcinoma
Joint Authors
Tang, Xiaofeng
Zhang, Weichen
Ye, Yufu
Li, Hong
Cheng, Longyu
Zhang, Min
Yu, Jun
Zheng, Shusen
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Sorafenib is a multi-target kinase inhibitor that has been approved as a unique target drug for the treatment of advanced hepatocellular carcinoma (HCC).
However, due to the frequent occurrence of drug resistance, its treatment efficacy is often limited.
The aim of this study was to explore the function of HOX transcript antisense intergenic RNA (HOTAIR) for the treatment of HCC with sorafenib, and its underlying mechanism.
Methods.
A cell counting kit-8 (CCK-8) assay and Edu assay were used to examine the viability and proliferation of HCC cells.
Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of HOTAIR and miR-217 in HCC cells.
Small interfering (si) RNA was transfected to knockdown HOTAIR to explore its biological function.
A Western blot and immunofluorescence were performed to detect the level of E-cadherin and Vimentin expression.
Results.
Sorafenib resistance was increased in HCC cells with high HOTAIR expression.
Moreover, a knockdown of HOTAIR could improve the therapeutic effect of sorafenib on HCC via increasing E-cadherin and decreasing Vimentin expression.
Additionally, a HOTAIR knockdown could increase the sensitivity of sorafenib for HCC treatment by up-regulating miR-217.
Conclusions.
Lnc HOTAIR could increase sorafenib resistance in HCC by inhibiting miR-217.
Our research attempts to elucidate a more effective treatment and provides novel insight into potential clinical treatment for HCC.
American Psychological Association (APA)
Tang, Xiaofeng& Zhang, Weichen& Ye, Yufu& Li, Hong& Cheng, Longyu& Zhang, Min…[et al.]. 2020. LncRNA HOTAIR Contributes to Sorafenib Resistance through Suppressing miR-217 in Hepatic Carcinoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138150
Modern Language Association (MLA)
Tang, Xiaofeng…[et al.]. LncRNA HOTAIR Contributes to Sorafenib Resistance through Suppressing miR-217 in Hepatic Carcinoma. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1138150
American Medical Association (AMA)
Tang, Xiaofeng& Zhang, Weichen& Ye, Yufu& Li, Hong& Cheng, Longyu& Zhang, Min…[et al.]. LncRNA HOTAIR Contributes to Sorafenib Resistance through Suppressing miR-217 in Hepatic Carcinoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138150
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138150