Lack of PPARβδ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis
Joint Authors
Shen, Bo
Li, Aimin
Wan, Yu-Jui Yvonne
Shen, Guijia
Zhu, Jinshui
Nie, Yuqiang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
The present study examined the role of PPARβ/δ in hepatocellular carcinoma (HCC).
Methods.
The effect of PPARβ/δ on HCC development was analyzed using PPARβ/δ-overexpressed liver cancer cells and PPARβ/δ-knockout mouse models.
Results.
PPARβ/δ(-/-) mice were susceptible to diethylnitrosamine- (DEN-) induced HCC (87.5% vs.
37.5%, p<0.05).
In addition, PPARβ/δ-overexpressed HepG2 cells had reduced proliferation, migration, and invasion capabilities accompanied by increased apoptosis and cell cycle arrest at the G0/G1 phase.
Moreover, differential gene expression profiling uncovered that the levels of serine/threonine-protein kinase (SGK-1) mRNA and its encoded protein were reduced in PPARβ/δ-overexpressed HepG2 cells.
Consistently, elevated SGK-1 levels were found in PPARβ/δ(-/-) mouse livers as well as PPARβ/δ-knockdown human SMMC-7721 HCC cells.
Chromatin immunoprecipitation (ChIP) assays followed by real-time quantitative polymerase chain reaction (qPCR) assays further revealed the binding of PPARβ/δ to the SGK-1 regulatory region in HepG2 cells.
Conclusions.
Due to the known tumor-promoting effect of SGK1, the present data suggest that PPARβ/δ-deactivated SGK1 is a novel pathway for inhibiting liver carcinogenesis.
American Psychological Association (APA)
Shen, Bo& Li, Aimin& Wan, Yu-Jui Yvonne& Shen, Guijia& Zhu, Jinshui& Nie, Yuqiang. 2020. Lack of PPARβδ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1138171
Modern Language Association (MLA)
Shen, Bo…[et al.]. Lack of PPARβδ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1138171
American Medical Association (AMA)
Shen, Bo& Li, Aimin& Wan, Yu-Jui Yvonne& Shen, Guijia& Zhu, Jinshui& Nie, Yuqiang. Lack of PPARβδ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1138171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138171