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Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A
Joint Authors
You, Liyi
Chen, Hao
Xu, Lixin
Li, Xun
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-07
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Objective.
Increasing evidence highlights the significance of microRNAs (miRNAs) in the progression of atherosclerosis (AS).
Our aim was to probe out the role and regulatory mechanism of miR-29a-3p in AS.
Methods.
An in vivo model of AS was conducted by high-fat diet ApoE-/- mice.
Oxidized low-density lipoprotein- (ox-LDL-) exposed vascular smooth muscle cells (VSMCs) were utilized as an in vitro of AS.
Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were detected.
Hematoxylin and eosin (H&E) and Masson’s staining was presented to investigate the pathological changes.
miR-29a-3p and TNFRSF1A expression was detected by RT-qPCR.
Proliferative, migrated, and invaded abilities of VSMCs were determined via a series of assays.
The interaction between miR-29a-3p and TNFRSF1A was verified through luciferase reporter assay.
Results.
Upregulated miR-29a-3p and downregulated TNFRSF1A were found both in vitro and in vivo models of AS.
miR-29a-3p mimic distinctly decreased the serum levels of TC, TG, and LDL-C and increased serum HDL-C levels.
Moreover, its overexpression could ameliorate plaque formation of AS mice.
In ox-LDL-induced VSMCs, miR-29a-3p overexpression notably decreased cell proliferation, migration, and invasion, which was reversed by TNFRSF1A overexpression.
Also, miR-29a-3p could directly target the 3′UTR of TNFRSF1A.
Conclusion.
miR-29a-3p overexpression ameliorated plaque formation of AS and suppressed proliferation, migration, and invasion of ox-LDL-induced VSMCs via TNFRSF1A, which offered novel insights into the progression of AS.
American Psychological Association (APA)
You, Liyi& Chen, Hao& Xu, Lixin& Li, Xun. 2020. Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1138205
Modern Language Association (MLA)
You, Liyi…[et al.]. Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1138205
American Medical Association (AMA)
You, Liyi& Chen, Hao& Xu, Lixin& Li, Xun. Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1138205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138205