Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRHLH Secretion during the Follicular Phase of the Estrous Cycle
Joint Authors
Herman, Anna
Herman, Andrzej Przemysław
Krawczyńska, Agata
Skipor, J.
Romanowicz, Katarzyna
Bochenek, Joanna
Wojtulewicz, Karolina
Paczesna, Kamila
Antushevich, Hanna
Tomaszewska-Zaremba, Dorota
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The study was designed to test the hypothesis that the inhibition of acetylcholinesterase (AChE) activity at the periphery by Neostigmine (0.5 mg/animal) will be sufficient to prevent inflammatory dependent suppression of the gonadotropin-releasing hormone (GnRH)/luteinising hormone (LH) secretion in ewes in the follicular phase of the estrous cycle, and this effect will be comparable with the systemic AChE inhibitor, Donepezil (2.5 mg/animal).
An immune/inflammatory challenge was induced by peripheral administration of lipopolysaccharide (LPS; 400 ng/kg).
Peripheral treatment with Donepezil and Neostigmine prevented the LPS-induced decrease (P<0.05) in LHβ gene expression in the anterior pituitary gland (AP) and in LH release.
Moreover, Donepezil completely abolished (P<0.05) the suppressory effect of inflammation on GnRH synthesis in the preoptic area, when pretreatment with Neostigmine reduced (P<0.05) the decrease in GnRH content in this hypothalamic structure.
Moreover, administration of both AChE inhibitors diminished (P<0.05) the inhibitory effect of LPS treatment on the expression of GnRH receptor in the AP.
Our study shows that inflammatory dependent changes in the GnRH/LH secretion may be eliminated or reduced by AChE inhibitors suppressing inflammatory reaction only at the periphery such as Neostigmine, without the need for interfering in the central nervous system.
American Psychological Association (APA)
Herman, Andrzej Przemysław& Skipor, J.& Krawczyńska, Agata& Bochenek, Joanna& Wojtulewicz, Karolina& Antushevich, Hanna…[et al.]. 2017. Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRHLH Secretion during the Follicular Phase of the Estrous Cycle. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1138234
Modern Language Association (MLA)
Herman, Andrzej Przemysław…[et al.]. Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRHLH Secretion during the Follicular Phase of the Estrous Cycle. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1138234
American Medical Association (AMA)
Herman, Andrzej Przemysław& Skipor, J.& Krawczyńska, Agata& Bochenek, Joanna& Wojtulewicz, Karolina& Antushevich, Hanna…[et al.]. Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRHLH Secretion during the Follicular Phase of the Estrous Cycle. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1138234
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138234