miR-375 Inhibits the Proliferation and Invasion of Nasopharyngeal Carcinoma Cells by Suppressing PDK1
Joint Authors
Jia-yuan, Xu
Wei, Song
Fang-fang, Lu
Zhi-jian, Dai
Long-he, Cao
Sen, Lin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
In patients with nasopharyngeal carcinoma (NPC), the expression of PDK1 is remarkably improved in NPC tissue and correlated with the clinicopathological severity of NPC.
We expressed miR-375 in NPC cells to study the effects on PDK1 gene expression.
We also investigated the mechanism by which miR-375 affects the biological behavior of NPC cells through effects on PDK1.
Methods.
qRT-PCR was carried out to analyze miR-375 and PDK1 levels in NPC cells.
NPC cells were transfected with miR-375 inhibitor or miR-375 mimic.
CCK-8 testing, colony formation testing, transwell testing, and flow cytometry analysis were carried out to quantify the cells’ biological behavior.
Rescue experiments demonstrated that the recovery of PDK1 expression was able to reverse the influence of miR-375 inhibition on NPC diffusion and intrusion.
The interaction between miR-375 and PDK1 was verified by dual-luciferase reporter gene testing.
Results.
The results revealed that miR-375 has a negative regulatory effect on PDK1 expression in NPC cells.
Furthermore, PDK1 is a target gene for miR-375.
The empirical results obtained demonstrated a negative correlation between tumor development and the level of miR-375 expression in NPC tissues.
The excessive expression of miR-375 and the downregulation of PDK1 facilitated the diffusion and invasion of NPC cells.
Conclusion.
The diffusion and incursion of NPC cells may be inhibited by direct targeting of PDK1 and decreasing the expression of miR-375.
Our study highlights efforts to target PDK1 and miR-375 as potential therapeutic strategies for use in the treatment of NPC.
American Psychological Association (APA)
Jia-yuan, Xu& Wei, Song& Fang-fang, Lu& Zhi-jian, Dai& Long-he, Cao& Sen, Lin. 2020. miR-375 Inhibits the Proliferation and Invasion of Nasopharyngeal Carcinoma Cells by Suppressing PDK1. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138239
Modern Language Association (MLA)
Jia-yuan, Xu…[et al.]. miR-375 Inhibits the Proliferation and Invasion of Nasopharyngeal Carcinoma Cells by Suppressing PDK1. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1138239
American Medical Association (AMA)
Jia-yuan, Xu& Wei, Song& Fang-fang, Lu& Zhi-jian, Dai& Long-he, Cao& Sen, Lin. miR-375 Inhibits the Proliferation and Invasion of Nasopharyngeal Carcinoma Cells by Suppressing PDK1. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138239
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138239