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Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
Joint Authors
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Durani, Lina Wati
Tan, Jen Kit
Chua, Kien Hui
Khor, Shy Cian
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Piper betle (PB) is a traditional medicine that is widely used to treat different diseases around Asian region.
The leaf extracts contain various bioactive compounds, which were reported to have antidiabetic, antibacterial, anti-inflammatory, antioxidant, and anticancer effects.
In this study, the effect of PB aqueous extracts on replicative senescent human diploid fibroblasts (HDFs) was investigated by determining the expressions of senescence-associated genes using quantitative PCR.
Our results showed that PB extracts at 0.4 mg/ml can improve cell proliferation of young (143%), presenescent (127.3%), and senescent (157.3%) HDFs.
Increased expressions of PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were observed in senescent HDFs compared to young and/or presenescent HDFs.
Treatment with PB extracts modulates the transcriptional profile changes in senescent HDFs.
By contrast, expressions of SOD1 increased, whereas GPX1, PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were decreased in PB-treated senescent HDFs compared to untreated senescent HDFs.
In conclusion, this study indicates the modulation of PB extracts on senescence-associated genes expression of replicative senescent HDFs.
Further studies warrant determining the mechanism of PB in modulating replicative senescence of HDFs through these signaling pathways.
American Psychological Association (APA)
Durani, Lina Wati& Khor, Shy Cian& Tan, Jen Kit& Chua, Kien Hui& Mohd Yusof, Yasmin Anum& Makpol, Suzana. 2017. Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138264
Modern Language Association (MLA)
Durani, Lina Wati…[et al.]. Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1138264
American Medical Association (AMA)
Durani, Lina Wati& Khor, Shy Cian& Tan, Jen Kit& Chua, Kien Hui& Mohd Yusof, Yasmin Anum& Makpol, Suzana. Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138264
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138264