The Expression and Potential Role of Tubulin Alpha 1b in Wilms’ Tumor

Joint Authors

Xu, Qiong-Qian
Qin, Li-Ting
Liang, Song-Wu
Chen, Peng
Gu, Jin-Han
Huang, Zhi-Guang
Yang, Xia
Gao, Li
Wang, Shi-Shuo
Luo, Yi-Ge
Liu, Lin-Le-Yi
Wang, Jun
Lin, Jin-Yan
Chen, Gang
Chen, Jia-Bo

Source

BioMed Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-25

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

We explored the difference in expression of tubulin alpha 1b (TUBA1B) between Wilms’ tumor (WT) and normal tissues (NT) from in-house patients and databases, to determine TUBA1B expression in WT and the predictive pathways of coexpressed genes.

In-house RNA-sequencing data were performed with WT and NT from three patients from our institute.

Other four RNA-sequencing and microarray data were also downloaded from multiple public databases.

The TUBA1B expression between WT and NT was analyzed by Student’s t-test and meta-analysis.

The correlation between the expression of TUBA1B and other genes in each study was analyzed.

Genes with p<0.05 and r>0.5 were considered as the coexpressing genes of TUBA1B.

Overlapping the coexpressed genes of the five studies, including three in-house patients (3 WT vs.

3 NT), GTEx-TARGET (126 WT vs.

51 NT), GSE2172 (18 WT vs.

3 NT), GSE11024 (27 WT vs.

12 NT), and GSE73209 (32 WT vs.

6 NT), were performed with limma and VennDiagram packages in R software.

The website of WEB-based GEne SeT AnaLysis toolkit were used to analyze the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotations for the overlapped genes.

The results showed that the relative expression of TUBA1B in WT tissues from in-house three patients was 280.0086, 141.7589, and 303.8292 and that in NT was 16.5836, 104.8141, and 12.79 (3 WT vs.

3 NT, p=0.0285, ROC=100%, SMD=2.74).

Student’s t-test and meta-analysis in all studies revealed that the expression of TUBA1B was upregulated in WT tissues compared to that in NT (p<0.05, SMD=2.89, sROC=0.98).

Finally, the research identified the expression of TUBA1B in WT tissues was significantly upregulated than that in NT.

The coexpressed genes of TUBA1B were enriched in the pathway of DNA replication, mismatch repair, cell cycle, pathogenic Escherichia coli infection, and spliceosome.

American Psychological Association (APA)

Xu, Qiong-Qian& Qin, Li-Ting& Liang, Song-Wu& Chen, Peng& Gu, Jin-Han& Huang, Zhi-Guang…[et al.]. 2020. The Expression and Potential Role of Tubulin Alpha 1b in Wilms’ Tumor. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138306

Modern Language Association (MLA)

Xu, Qiong-Qian…[et al.]. The Expression and Potential Role of Tubulin Alpha 1b in Wilms’ Tumor. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1138306

American Medical Association (AMA)

Xu, Qiong-Qian& Qin, Li-Ting& Liang, Song-Wu& Chen, Peng& Gu, Jin-Han& Huang, Zhi-Guang…[et al.]. The Expression and Potential Role of Tubulin Alpha 1b in Wilms’ Tumor. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138306

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1138306