Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated
Joint Authors
Xu, Mengmeng
Che, Long
Yang, Zhenguo
Zhang, Pan
Shi, Jiankai
Li, Jian
Lin, Yan
Fang, Zhengfeng
Che, Lianqiang
Feng, Bin
Wu, De
Xu, Shengyu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Primordial follicle formation represents a critical phase of the initiation of embryonic reproductive organ development, while the primordial follicle transition into primary follicle determines whether oestrus or ovulation will occur in female animals.
To identify molecular mechanism of new proteins which are involved in ovarian development, we employed 2D-DIGE to compare the protein expression profiles of primordial follicles and primary follicles of fetal ovaries in pigs.
Fetal ovaries were collected at distinct time-points of the gestation cycle (g55 and g90).
The identified proteins at the g55 time-point are mainly involved in the development of anatomical structures [reticulocalbin-1 (RCN1), reticulocalbin-3 (RCN3)], cell differentiation (actin), and stress response [heterogeneous nuclear ribonucleoprotein K (HNRNPK)].
Meanwhile, at the g90 stage, the isolated proteins with altered expression levels were mainly associated with cell proliferation [major vault protein (MVP)] and stress response [heat shock-related 70 kDa protein 2 (HSPA2)].
In conclusion, our work revealed that primordial follicle formation is regulated by RCN1, RCN3, actin, and HNRNPK, while the primordial follicle transformation to primary follicle is regulated by MVP and HSPA2.
Therefore, our results provide further information for the prospective understanding of the molecular mechanism(s) involved in the regulation of the ovarian follicle development.
American Psychological Association (APA)
Xu, Mengmeng& Che, Long& Yang, Zhenguo& Zhang, Pan& Shi, Jiankai& Li, Jian…[et al.]. 2017. Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1138318
Modern Language Association (MLA)
Xu, Mengmeng…[et al.]. Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1138318
American Medical Association (AMA)
Xu, Mengmeng& Che, Long& Yang, Zhenguo& Zhang, Pan& Shi, Jiankai& Li, Jian…[et al.]. Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1138318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138318