Mining of Microbial Genomes for the Novel Sources of Nitrilases
Joint Authors
Thakur, Neerja
Raj, Tilak
Savitri, Tilak
Sharma, Nikhil
Bhalla, Tek Chand
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Next-generation DNA sequencing (NGS) has made it feasible to sequence large number of microbial genomes and advancements in computational biology have opened enormous opportunities to mine genome sequence data for novel genes and enzymes or their sources.
In the present communication in silico mining of microbial genomes has been carried out to find novel sources of nitrilases.
The sequences selected were analyzed for homology and considered for designing motifs.
The manually designed motifs based on amino acid sequences of nitrilases were used to screen 2000 microbial genomes (translated to proteomes).
This resulted in identification of one hundred thirty-eight putative/hypothetical sequences which could potentially code for nitrilase activity.
In vitro validation of nine predicted sources of nitrilases was done for nitrile/cyanide hydrolyzing activity.
Out of nine predicted nitrilases, Gluconacetobacter diazotrophicus, Sphingopyxis alaskensis, Saccharomonospora viridis, and Shimwellia blattae were specific for aliphatic nitriles, whereas nitrilases from Geodermatophilus obscurus, Nocardiopsis dassonvillei, Runella slithyformis, and Streptomyces albus possessed activity for aromatic nitriles.
Flavobacterium indicum was specific towards potassium cyanide (KCN) which revealed the presence of nitrilase homolog, that is, cyanide dihydratase with no activity for either aliphatic, aromatic, or aryl nitriles.
The present study reports the novel sources of nitrilases and cyanide dihydratase which were not reported hitherto by in silico or in vitro studies.
American Psychological Association (APA)
Sharma, Nikhil& Thakur, Neerja& Raj, Tilak& Savitri, Tilak& Bhalla, Tek Chand. 2017. Mining of Microbial Genomes for the Novel Sources of Nitrilases. BioMed Research International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1138344
Modern Language Association (MLA)
Sharma, Nikhil…[et al.]. Mining of Microbial Genomes for the Novel Sources of Nitrilases. BioMed Research International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1138344
American Medical Association (AMA)
Sharma, Nikhil& Thakur, Neerja& Raj, Tilak& Savitri, Tilak& Bhalla, Tek Chand. Mining of Microbial Genomes for the Novel Sources of Nitrilases. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1138344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138344