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Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae
Joint Authors
Tan, Qi
Bao, Da Peng
Li, Yan
Wang, Ying
Gao, Yingnv
Wang, Ying
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
High-throughput technologies of functional genomics such as T-DNA insertional mutagenesis and microarray expression profiling have been employed to identify genes related to pathogenicity in Magnaporthe oryzae.
However, validation of the functions of individual genes identified by these high-throughput approaches is laborious.
In this study, we compared two published lists of genes putatively related to pathogenicity in M.
oryzae identified by T-DNA insertional mutagenesis (comprising 1024 genes) and microarray expression profiling (comprising 236 genes), respectively, and then validated the functions of some overlapped genes between the two lists by knocking them out using the method of target gene replacement.
Surprisingly, only 13 genes were overlapped between the two lists, and none of the four genes selected from the overlapped genes exhibited visible phenotypic changes on vegetative growth, asexual reproduction, and infection ability in their knockout mutants.
Our results suggest that both of the lists might contain large proportions of unrelated genes to pathogenicity and therefore comparing the two gene lists is hardly helpful for the identification of genes that are more likely to be involved in pathogenicity as we initially expected.
American Psychological Association (APA)
Wang, Ying& Wang, Ying& Tan, Qi& Gao, Yingnv& Li, Yan& Bao, Da Peng. 2017. Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1138442
Modern Language Association (MLA)
Wang, Ying…[et al.]. Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1138442
American Medical Association (AMA)
Wang, Ying& Wang, Ying& Tan, Qi& Gao, Yingnv& Li, Yan& Bao, Da Peng. Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1138442
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138442