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miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway
Joint Authors
Xiang, Yaoyv
Li, Yanlin
Yang, Lingjian
He, Yinghong
Jia, Di
Hu, Xidan
Source
Biochemistry Research International
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-25
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Osteoarthritis (OA) is a chronic joint function disorder with characteristics of chondrocytes reduction and extracellular matrix (ECM) components destruction.
MicroRNAs (miRNAs) and the SDF-1/CXCR4 axis are essential factors of chondrocyte apoptosis and ECM degeneration.
However, very few studies have investigated the correlation between miRNAs and the SDF-1/CXCR4 axis in osteoarthritis so far.
Here, through miRNAs microarray and bioinformatics analyses, we identified miR-142-5p as a CXCR4-targeted and dramatically downregulated miRNA in cartilage from OA patients, as well as in SDF-1-induced OA chondrocytes in vitro.
In SDF-1-treated primary human OA chondrocytes that were transfected with a miR-142-5p mimic or inhibitor, the expression of CXCR4 was found to be inversely correlated with the expression of miR-142-5p.
The dual luciferase reporter assay further verified the target relationship between miR-142-5p and CXCR4.
Overexpression of miR-142-5p alleviated OA pathology by suppressing chondrocyte apoptosis, even in CXCR4 overexpressed OA chondrocytes.
This was associated with decreased cartilage matrix degradation, reduced cartilage inflammation, and inactivated MAPK signaling pathway.
Our study suggests that upregulated expression of CXCR4-targeted miR-142-5p can inhibit apoptosis, inflammation, and matrix catabolism and inactivate the MAPK signaling pathway in OA chondrocytes.
Our work provides important insight into targeting miR-142-5p and the SDF-1/CXCR4 axis in OA therapy.
American Psychological Association (APA)
Xiang, Yaoyv& Li, Yanlin& Yang, Lingjian& He, Yinghong& Jia, Di& Hu, Xidan. 2020. miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway. Biochemistry Research International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1138448
Modern Language Association (MLA)
Xiang, Yaoyv…[et al.]. miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway. Biochemistry Research International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1138448
American Medical Association (AMA)
Xiang, Yaoyv& Li, Yanlin& Yang, Lingjian& He, Yinghong& Jia, Di& Hu, Xidan. miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway. Biochemistry Research International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1138448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138448